Alteration of the ATG start codon of the A* protein of bacteriophage [phi]X174 into an ATT codon yields a viable phage indicating that A* protein is not essential for [phi]X174 reproduction
| Autor: | H.A.A.M. van Teeffelen, A.D.M. van Mansfeld, H. Liewerink, J. H. Van Boom, H.S. Jansz, P.D. Baas |
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| Jazyk: | angličtina |
| Rok vydání: | 1987 |
| Předmět: |
RFC5
DNA Replication ATG start codon viruses Mutant Biophysics Bacteriophage phi X174 Virus Replication Biochemistry Bacteriophage DDB1 Viral Proteins Start codon (Bacteriophage ϕX174) Structural Biology Genetics Rolling circle DNA replication RNA Messenger oligonucleotide-directed mutagenesis Codon Molecular Biology Gene A* protein Endodeoxyribonucleases biology Base Sequence Cell Biology biology.organism_classification DNA-Binding Proteins (Bacteriophage [phi]X174) Mutation Biologie Bacteriophage phi X 174 |
| Zdroj: | FEBS Letters, 218(1), 119. Elsevier |
| ISSN: | 0014-5793 |
| Popis: | Bacteriophage phi X174 gene A encodes two proteins: the gene A protein and the smaller A protein, which is synthesized from a translational start signal within the A gene in the same reading frame as the gene A protein. The gene A protein is involved in initiation, elongation and termination of rolling circle DNA replication. The role of the A protein in the life cycle of phi X174, however, is unknown. Using oligonucleotide-directed mutagenesis a viable phi X174 mutant was constructed in which the ATG start codon of the A protein was changed into an ATT codon. This mutant, phi X-4499T, does not synthesize A protein. The burst size of phi X-4499T amounted to 50% of that of wild type phi X174. This indicates that A protein, although advantageous for phage reproduction, is not essential during the life cycle of bacteriophage phi X174. |
| Databáze: | OpenAIRE |
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