Detection of aneuploidy by array comparative genomic hybridization using cell lines to mimic a mosaic trophectoderm biopsy
| Autor: | Joyce C. Harper, Anthony Gordon, Anthony Brown, T Mamas, Sioban SenGupta |
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| Rok vydání: | 2011 |
| Předmět: |
Biopsy
Aneuploidy Biology Cell Line Predictive Value of Tests Ectoderm medicine Chromosomes Human Humans Blastocyst Genetic Testing Preimplantation Diagnosis Genetics Comparative Genomic Hybridization medicine.diagnostic_test Mosaicism Obstetrics and Gynecology Embryo medicine.disease Molecular biology Trophoblasts medicine.anatomical_structure Reproductive Medicine Cell culture embryonic structures Female Ploidy Trophectoderm biopsy Comparative genomic hybridization |
| Zdroj: | Fertility and sterility. 97(4) |
| ISSN: | 1556-5653 |
| Popis: | Objective To examine the effect of mosaicism in the array comparative genomic hybridization result during preimplantation genetic screening after blastocyst biopsy. Design Experimental study. Setting University laboratory. Material(s) Epithelial cell lines. Intervention(s) Mixing of euploid and aneuploid cells to create mosaic trophectoderm and blastocyst models. Main Outcome Measure(s) The level of aneuploidy in samples with different ratios of aneuploid cells was measured after array comparative genomic hybridization. Result(s) A shift from normality was present when the level of aneuploid cells in the sample was >25%. Aneuploidy could be confidently called when the level of aneuploid cells was >50%. Conclusion(s) This study determined that aneuploidy in mosaic samples can be detected by array comparative genomic hybridization and that the result may also indicate the proportion of the aneuploid cells present in the sample. |
| Databáze: | OpenAIRE |
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