Bioactive Coating with Two-Layer Hierarchy of Relief Obtained by Sol-Gel Method with Shock Drying and Osteoblast Response of Its Structure
| Autor: | E. G. Zemtsova, Vladimir M. Smirnov, Ruslan Z. Valiev, Andrei Yu. Arbenin, Natalia M. Yudintceva, Evgeniy V. Orekhov |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
implant General Chemical Engineering 02 engineering and technology engineering.material 010402 general chemistry 01 natural sciences Dip-coating Osseointegration Article lcsh:Chemistry Coating composite sol-gel dip coating two-level hierarchy of relief bioactivity osseointegration titania osteoblast medicine General Materials Science Composite material Sol-gel Nanoporous Osteoblast 021001 nanoscience & nanotechnology 0104 chemical sciences Shock (mechanics) medicine.anatomical_structure lcsh:QD1-999 engineering 0210 nano-technology Layer (electronics) |
| Zdroj: | Nanomaterials Nanomaterials, Vol 7, Iss 10, p 323 (2017) Nanomaterials; Volume 7; Issue 10; Pages: 323 |
| ISSN: | 2079-4991 |
| Popis: | In this work, we analyze the efficiency of the modification of the implant surface. This modification was reached by the formation of a two-level relief hierarchy by means of a sol-gel approach that included dip coating with subsequent shock drying. Using this method, we fabricated a nanoporous layer with micron-sized defects on the nanotitanium surface. The present work continues an earlier study by our group, wherein the effect of osteoblast-like cell adhesion acceleration was found. In the present paper, we give the results of more detailed evaluation of coating efficiency. Specifically, cytological analysis was performed that included the study of the marker levels of osteoblast-like cell differentiation. We found a significant increase in the activity of alkaline phosphatase at the initial incubation stage. This is very important for implantation, since such an effect assists the decrease in the induction time of implant engraftment. Moreover, osteopontin expression remains high for long expositions. This indicates a prolonged osteogenic effect in the coating. The results suggest the acceleration of the pre-implant area mineralization and, correspondingly, the potential use of the developed coatings for bone implantation. |
| Databáze: | OpenAIRE |
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