Hydroxyl Radical as a Potential Intracellular Mediator of Polymorphonuclear Neutrophil Apoptosis
| Autor: | Marie-José Grange, Catherine Pasquier, Emmanuelle Rollet-Labelle, Claude Marquetty, Carole Elbim, Marie-Anne Gougerot-Pocidalo |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Xanthine Oxidase
Neutrophils Iron Apoptosis DNA Fragmentation Iron Chelating Agents medicine.disease_cause Biochemistry Superoxide dismutase Glucose Oxidase chemistry.chemical_compound Superoxides Physiology (medical) medicine Humans Buthionine sulfoximine Hydrogen peroxide Xanthine oxidase chemistry.chemical_classification Reactive oxygen species biology Hydroxyl Radical Superoxide Dismutase Chemistry Superoxide Hydrogen Peroxide Catalase Flow Cytometry Glutathione biology.protein Oxidation-Reduction Oxidative stress |
| Zdroj: | Free Radical Biology and Medicine. 24:563-572 |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/s0891-5849(97)00292-x |
| Popis: | We investigated reactive oxygen species (ROS) involvement in polymorphonuclear neutrophilic leukocyte (neutrophil) apoptosis triggering. Neutrophils were incubated with xanthine oxidase (XO), which produces superoxide anion (O2.-) and hydrogen peroxide (H2O2) or glucose oxidase (GO), which produces only H2O2. Both XO and GO accelerated apoptosis when compared to spontaneously aged neutrophils. Catalase inhibited both spontaneous apoptosis and XO- or GO-accelerated apoptosis, but superoxide dismutase did not. Hydrogen peroxide can enter the cell, thus generating intracellular oxidation, which was observed by flow cytometry. Furthermore, the intracellular reduced glutathione content fell in the presence of XO or GO; however, apoptosis was not accelerated in the presence of buthionine sulfoximine (BSO), suggesting that the fall in glutathione in the presence of XO or GO is a consequence of oxidative stress but not a trigger of apoptosis. Hydrogen peroxide can react with iron to form hydroxyl radicals (HO.); we observed that two iron chelators, deferoxamine and hydroxybenzyl ethylenediamine (HBED), both inhibited spontaneous and accelerated apoptosis, suggesting that HO. may mediate neutrophil apoptosis. |
| Databáze: | OpenAIRE |
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