Identification of lncRNA-associated competing endogenous RNA networks for occurrence and prognosis of gastric carcinoma
| Autor: | Wumin Jin, Lianmin Ye |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Clinical Biochemistry Gastric carcinoma Computational biology Biology Stomach Neoplasms Cell Line Tumor microRNA medicine Immunology and Allergy Humans competing endogenous RNA Gene Regulatory Networks RNA Messenger Research Articles Critical pathways Competing endogenous RNA gastric cancer Biochemistry (medical) Carcinoma Public Health Environmental and Occupational Health Cancer RNA Hematology bioinformatics Oncogenes medicine.disease Prognosis Gene Expression Regulation Neoplastic Medical Laboratory Technology MicroRNAs Identification (biology) RNA Long Noncoding Research Article |
| Zdroj: | Journal of Clinical Laboratory Analysis |
| DOI: | 10.21203/rs.3.rs-45596/v1 |
| Popis: | Background Gastric cancer (GC) is one of the common digestive malignancies worldwide and causes a severe public health issue. So far, the underlying mechanisms of GC are largely unclear. Thus, we aim to identify the long non‐coding RNA (lncRNA)‐associated competing endogenous RNA (ceRNA) for GC. Methods TCGA database was downloaded and used for the identification of differentially expressed (DE) lncRNAs, miRNAs, and mRNAs, respectively. Then, the ceRNA network was constructed via multiple online datasets and approaches. In addition, various in vitro assays were carried out to validate the effect of certain hub lncRNAs. Results We constructed a ceRNA network, including 76 lncRNAs, 18 miRNAs, and 159 mRNAs, which involved multiple critical pathways. Next, univariate and multivariate analysis demonstrated 11 lncRNAs, including LINC02731, MIR99AHG, INHBA‐AS1, CCDC144NL‐AS1, VLDLR‐AS1, LIFR‐AS1, A2M‐AS1, LINC01537, and LINC00702, and were associated with OS, and nine of those lncRNAs were considered as hub lncRNAs involved in the sub‐ceRNA network. The in vitro assay indicated two lncRNAs, INHBA‐AS1 and CCDC144NL‐AS1, which were positively related to the GC aggressive features, including proliferation, invasion, and migration. Conclusions We identified nine hub lncRNAs and the associated ceRNA network related to the prognosis of GC, and then validated two out of them as promising oncogenes in GC. So far, the underlying mechanisms of GC are largely unclear. The competing endogenous RNA (ceRNA) network has been proved to display a critical role in tumorigenesis and progression. The present study is to identify the long non‐coding RNA (lncRNA) associated ceRNA for GC. We identified differentially expressed (DE) lncRNAs, miRNAs and mRNAs based on TCGA database. Then, the ceRNA network was constructed via multiple online datasets and approaches. Furthermore, 9 lncRNAs were considered as hub lncRNAs involved in sub‐ceRNA network via survival analysis. Subsequently, in vitro assay indicated 2 lncRNAs, INHBA‐AS1 and CCDC144NL‐AS1, were positively related to the proliferation, invasion, and migration in GC. This indicates they are promising oncogenes in GC. |
| Databáze: | OpenAIRE |
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