The suppression of osmoinduced proline response of Brassica napus L. var oleifera leaf discs by polyunsaturated fatty acids and methyl-jasmonate

Autor: François Larher, Corinne Lefort, Valérie Maerskalck, Neil R. J. Emery, Ronan Sulpice, Valérie Huguet-Robert
Přispěvatelé: Interactions Cellulaires et Moléculaires, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-IFR98-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-IFR98-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2003
Předmět:
0106 biological sciences
Osmotic shock
Proline
Linoleic acid
Water stress
Plant Science
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Lipoxygenase
Genetics
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
Fatty acids
Abscisic acid
Methyl-jasmonate
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
Methyl jasmonate
biology
Brassica napus
food and beverages
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
General Medicine
Oxylipin
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biomolecules [q-bio.BM]
chemistry
Biochemistry
biology.protein
lipids (amino acids
peptides
and proteins)
Agronomy and Crop Science
010606 plant biology & botany
Polyunsaturated fatty acid
Zdroj: Plant Science
Plant Science, Elsevier, 2003, 164 (1), pp.119-127. ⟨10.1016/S0168-9452(02)00343-6⟩
Plant Science, 2003, 164 (1), pp.119-127. ⟨10.1016/S0168-9452(02)00343-6⟩
ISSN: 0168-9452
DOI: 10.1016/S0168-9452(02)00343-6⟩
Popis: When subjected in vitro to upshock osmotic stress, canola leaf discs accumulate large amounts of proline. In this study, we investigated the regulatory effect exerted on the osmoinduced proline response by polyunsaturated fatty acids (PUFAs) and derivatives of the oxylipin pathway. When supplied to the stressing medium, PUFAs were found to inhibit proline accumulation, with α and γ-linolenic acid (C18:3) being more effective than linoleic acid (C18:2). The amount of accumulated proline increased to 200 μmol g −1 dry weight (DW) and remained constant in leaf discs subjected for 8 h to a stress medium containing 350 g l −1 PEG 6000 added with PUFAs, compared with levels observed in control discs treated with PEG that continued to increase to approximately 600 μmol g −1 DW regularly for up to 24 h. The suppressing effect of PUFAs was mimicked by methyl-jasmonate (Me-JA). Whatever the mediator used, this effect was dependent on its concentration and on the moment when osmotic stress was applied: γ-C18:3>α-C18:3>C18:2. An identical effect was observed with 12-oxo-phytodienoic acid (12-OPDA). In addition, the suppressing effect of Me-JA was found to counteract the promoting effect of abscisic acid (ABA). Collectively, these results demonstrated that proline accumulation by canola leaf explants subjected in vitro to osmotic stress could be partly suppressed by exogenously supplied free fatty acids (FAs) and by products resulting from their hydroperoxidation by lipoxygenase (LOXs). The physiological relevance of these findings is discussed.
Databáze: OpenAIRE
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