Micro-RNA-96 and interleukin-10 are independent biomarkers for multiple sclerosis activity
| Autor: | Nawal F. Abdel Ghaffar, Hatem S Shehata, Nevin M. Shalaby, Eslam El-Nahrery, Reda Abd El Aal, Magdy M. Mohamed, Mohamed Salah Sedeeq, Mona Hussein |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Disease duration Gastroenterology Young Adult 03 medical and health sciences Multiple Sclerosis Relapsing-Remitting 0302 clinical medicine Internal medicine microRNA Humans Medicine In patient 030212 general & internal medicine business.industry Multiple sclerosis Case-control study medicine.disease Interleukin-10 MicroRNAs Interleukin 10 Real-time polymerase chain reaction Neurology Relapsing remitting Case-Control Studies Disease Progression Egypt Female Neurology (clinical) business Biomarkers 030217 neurology & neurosurgery |
| Zdroj: | Journal of the Neurological Sciences. 403:92-96 |
| ISSN: | 0022-510X |
| DOI: | 10.1016/j.jns.2019.06.022 |
| Popis: | Background Micro-RNAs (miRNAs) are evolving as biological markers for multiple sclerosis (MS) both in activity and remission. miR-96 is associated with remission, however, the exact mechanism through which it contributes to the anti-inflammatory pathway is not clear. Objective To study the expression of miR-96 and IL-10 (anti-inflammatory mediator) in relapsing remitting (RR) MS. Subjects and methods A case control study including 32 RRMS patients from Kasr Al-Ainy MS clinic, Cairo University, Egypt, and 26 healthy controls (HC). Assessment of serum IL-10 by ELISA, and miR-96 via real time PCR was done during relapse and remission in patients, and in HC. Results IL-10 was higher in RRMS patients during remission and in HC compared with relapse (P ˂ 0.001). miR-96 expression was higher in RRMS patients during remission compared with relapse and HC, and was higher in HC than in relapse (P ˂ 0.001). IL-10 level in remission correlated positively with disease duration (r = 0.41; P = 0.02). Otherwise, no correlation was found between IL-10 and relapse number or EDSS (P>0.05). miR-96 in relapse negatively correlated with EDSS in relapse (r=−0.47; P=0.007), but no correlation was found with disease duration or relapse number, whereas, miR-96 in remission did not correlate with any clinical parameters (P>0.05). No correlation was found between IL-10 and miR-96 either in relapse or remission (P>0.05). Conclusion IL-10 and miR-96 are associated with MS quiescence, however, the lack of a significant correlation between them implicates that the influence of miR-96 may be exhibited through some pathway other than IL-10. |
| Databáze: | OpenAIRE |
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