Dictyostelium amoebae lacking an F-box protein form spores rather than stalk in chimeras with wild type
| Autor: | Herbert L. Ennis, Stefan Pukatzki, Richard H. Kessin, Dee N. Dao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Mutant
Molecular Sequence Data Protozoan Proteins F-box protein Chimera (genetics) Skp1 Animals Dictyostelium Northern blot Amino Acid Sequence Gene DNA Primers Multidisciplinary biology Base Sequence Chimera F-Box Proteins fungi Wild type Biological Sciences Spores Fungal biology.organism_classification Molecular biology Phenotype Mutation biology.protein Carrier Proteins |
| Popis: | Using a selection for Dictyostelium mutants that preferentially form spores, we have recovered a mutant called CheaterA. In chimeras with isogenic wild-type cells, the CheaterA mutant preferentially forms viable spores rather than inviable stalk cells. The mutant causes wild-type cells that have begun to express spore-specific genes to accumulate in the prestalk compartment of the developing organism. In the wild-type cells, the chtA transcript is absent in growing cells and appears early in development. No transcript was detected in the mutant by Northern blot. The chtA gene codes for a protein with an F-box and WD40 domains. This class of protein usually forms part of an S kp1, c ullin, F -box (SCF) complex that targets specific protein substrates for ubiquitination and degradation. |
| Databáze: | OpenAIRE |
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