Carotid Chemoreceptor Reflex Modulation by Arginine-Vasopressin Microinjected into the Nucleus Tractus Solitarius in Rats

Autor: Mónica Lemus, Elena Roces de Álvarez-Buylla, Alvarez-Buylla R, Alexander Yarkov, Sergio Montero
Rok vydání: 2006
Předmět:
Zdroj: Archives of Medical Research. 37:709-716
ISSN: 0188-4409
DOI: 10.1016/j.arcmed.2006.03.001
Popis: Background In addition to their role of sensing O 2 , pH, CO 2 , osmolarity and temperature, carotid body receptors (CBR) were proposed by us and others to have a glucose-sensing role in the blood entering the brain, integrating information about blood glucose and O 2 levels essential for central nervous system (CNS) metabolism. The nucleus tractus solitarius (NTS) is an important relay station in central metabolic control and receives signals from peripheral glucose-sensitive hepatoportal afferences, from central glucose-responsive neurons in the brainstem and from CBR and arginine–vasopressin (AVP)-containing axons from hypothalamic nuclei. Methods In normal Wistar rats anesthetized with pentobarbital, permanent cannulas were placed stereotaxically in the NTS. Glucose changes were induced in vivo after CBR stimulation with sodium cyanide (NaCN-5 μg/100 g), preceded by an infusion of AVP [(10 or 40 pmol/100 nL of artificial cerebrospinal fluid) aCSF] or an antagonist for V1a receptors (anti-glycogenolytic vasopressin analogue-VP1-A) (100 pmol/100 nL of aCSF) into the NTS. Results CBR stimulation after an AVP infusion (larger dose) into the NTS resulted in a significantly higher arterial glucose and lower brain arterial–venous glucose difference. In the same way, VP1-A administration in the NTS significantly decreased the effects observed after AVP priming before CBR stimulation or preceding the CBR stimulation, alone. Conclusions We propose that AVP in the NTS could participate in glucose homeostasis, modulating the information arising in CBR after histotoxic-anoxia stimulation.
Databáze: OpenAIRE
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