| Popis: |
A molecularly imprinted polymer (MIP)-based electrochemical sensor featuring an electrochemically grafted para-phenylenediamine functional monomer on a reduced graphene oxide-gold nanoparticles composite modified screen printed electrode is reported. The morphology and properties of the sensing material were characterized with microscopy, spectroscopy and electrochemical techniques. A number of factors affecting the performance of the MIP sensor were examined and optimized. Under an optimized condition, the imprinted electrochemical sensor yielded homogenous naloxone binding sites with a dissociation constant of 8.6 μM, and responded linearly up to 8 μM naloxone, with a limit of detection of 0.16 μM. The sensor showed good run-to-run repeatability and batch-to-batch performance reproducibility with relative standard deviation of 5.7%–9.6% (n = 4) and |
