Modulation of imprinted gene expression following superovulation
| Autor: | Kirsten M. Niles, Jacquetta M. Trasler, Serge McGraw, Amanda L. Fortier, Mylène Landry, Flavia L. Lopes |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Placenta media_common.quotation_subject Superovulation Reproductive technology Biology Biochemistry Epigenesis Genetic Genomic Imprinting Mice Endocrinology Insulin-Like Growth Factor II Pregnancy Internal medicine medicine Animals Imprinting (psychology) Molecular Biology Ovulation media_common Fetus KCNQ1OT1 Pregnancy Outcome Embryo Organ Size DNA Methylation Embryo Mammalian Placentation Embryo transfer embryonic structures Female RNA Long Noncoding Genomic imprinting |
| Zdroj: | Molecular and Cellular Endocrinology. 388:51-57 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/j.mce.2014.03.003 |
| Popis: | Although assisted reproductive technologies increase the risk of low birth weight and genomic imprinting disorders, the precise underlying causes remain unclear. Using a mouse model, we previously showed that superovulation alters the expression of imprinted genes in the placenta at 9.5days (E9.5) of gestation. Here, we investigate whether effects of superovulation on genomic imprinting persisted at later stages of development and assess the surviving fetuses for growth and morphological abnormalities. Superovulation, followed by embryo transfer at E3.5, as compared to spontaneous ovulation (controls), resulted in embryos of normal size and weight at 14.5 and 18.5days of gestation. The normal monoallelic expression of the imprinted genes H19, Snrpn and Kcnq1ot1 was unaffected in either the placentae or the embryos from the superovulated females at E14.5 or E18.5. However, for the paternally expressed imprinted gene Igf2, superovulation generated placentae with reduced production of the mature protein at E9.5 and significantly more variable mRNA levels at E14.5. We propose that superovulation results in the ovulation of abnormal oocytes with altered expression of imprinted genes, but that the coregulated genes of the imprinted gene network result in modulated expression. |
| Databáze: | OpenAIRE |
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