Experimental studies on unconjugated bilirubin binding by human erythrocytes
| Autor: | B.Magnan de Bornier, M. Hayer, M.-T. Piva, V. Sieso |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Adult
Male Erythrocytes Bilirubin Clinical Biochemistry Biochemistry chemistry.chemical_compound medicine Humans Binding site Phospholipids Serum Albumin Dose-Response Relationship Drug Chemistry Biochemistry (medical) Erythrocyte Membrane General Medicine Middle Aged medicine.disease Human serum albumin Unconjugated bilirubin Dissociation constant Red blood cell medicine.anatomical_structure Kernicterus Human erythrocytes medicine.drug |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 186(3) |
| ISSN: | 0009-8981 |
| Popis: | The evaluation of the bilirubin bound to human erythrocytes is considered by some paediatricians as a test to estimate the risk of development of kernicterus. We have studied the physical and chemical characteristics of this binding. Red blood cell membranes contain specific binding sites for bilirubin, the affinity of which is low (Kd = 170 μmol/l). The dissociation constant of the bilirubin/human serum albumin complex is about 10000 times lower. In jaundiced neonates even with a level of blood bilirubin higher than 300 μmol/l, the binding of bilirubin to red blood cells is negligible. So, the evaluation of the bilirubin bound to human red blood cells does not seem to be a useful test to appreciate the risk of development of kernicterus. |
| Databáze: | OpenAIRE |
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