Broad-range lytic bacteriophages that kill Staphylococcus aureus local field strains
| Autor: | Soledad Telma Carrasco, Virginia Abatángelo, Cristian Alejandro Suárez, Carina Andrea Boncompain, Natalia Peressutti Bacci, Ariel Fernando Amadio, Héctor R. Morbidoni |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Phage display Staphylococcus Antibiotics lcsh:Medicine medicine.disease_cause Pathology and Laboratory Medicine purl.org/becyt/ford/1 [https] Database and Informatics Methods Phage Display Medicine and Health Sciences Bacteriophages Staphylococcus Aureus lcsh:Science Pathogen Viral Genomics Multidisciplinary BIOINFORMATICS STAPHYLOCOCCUS Genomics Genomic Databases Secuencia Nucleotídica Bacterial Pathogens Molecular Biology Display Techniques Lytic cycle Staphylococcus aureus Medical Microbiology Myoviridae Bacteriófagos Viruses Pathogens Sequence Analysis CIENCIAS NATURALES Y EXACTAS Research Article Methicillin-Resistant Staphylococcus aureus medicine.drug_class Bioinformatics Otras Ciencias Biológicas Genetic Vectors BACTERIOPHAGES Virulence Genome Viral Microbial Genomics Biology Research and Analysis Methods Microbiology Ciencias Biológicas 03 medical and health sciences Antibiotic resistance Microscopy Electron Transmission Vectores Genéticos Virology medicine Genetics purl.org/becyt/ford/1.6 [https] Molecular Biology Techniques Microbial Pathogens Molecular Biology Molecular Biology Assays and Analysis Techniques Bacteria lcsh:R Nucleotide Sequence Organisms Correction Biology and Life Sciences Computational Biology Sequence Analysis DNA Comparative Genomics Genome Analysis BIOCONTROL 030104 developmental biology Biological Databases lcsh:Q Sequence Alignment |
| Zdroj: | PLoS ONE CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET PLoS ONE, Vol 12, Iss 7, p e0181671 (2017) PLoS ONE 12 (7) : e0181671 (July 2017) INTA Digital (INTA) Instituto Nacional de Tecnología Agropecuaria instacron:INTA |
| ISSN: | 1932-6203 |
| Popis: | Staphylococcus aureus is a very successful opportunistic pathogen capable of causing a variety of diseases ranging from mild skin infections to life-threatening sepsis, meningitis and pneumonia. Its ability to display numerous virulence mechanisms matches its skill to display resistance to several antibiotics, including β-lactams, underscoring the fact that new anti-S. aureus drugs are urgently required. In this scenario, the utilization of lytic bacteriophages that kill bacteria in a genus -or even species- specific way, has become an attractive field of study. In this report, we describe the isolation, characterization and sequencing of phages capable of killing S. aureus including methicillin resistant (MRSA) and multi-drug resistant S. aureus local strains from environmental, animal and human origin. Genome sequencing and bio-informatics analysis showed the absence of genes encoding virulence factors, toxins or antibiotic resistance determinants. Of note, there was a high similarity between our set of phages to others described in the literature such as phage K. Considering that reported phages were obtained in different continents, it seems plausible that there is a commonality of genetic features that are needed for optimum, broad host range anti-staphylococcal activity of these related phages. Importantly, the high activity and broad host range of one of our phages underscores its promising value to control the presence of S. aureus in fomites, industry and hospital environments and eventually on animal and human skin. The development of a cocktail of the reported lytic phages active against S. aureus–currently under way- is thus, a sensible strategy against this pathogen. Fil: Abatángelo, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina Fil: Peressutti Bacci, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina Fil: Boncompain, Carina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina Fil: Amadio, Ariel Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Carrasco, Soledad Telma. Universidad Nacional de Rosario; Argentina Fil: Suárez, Cristian Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina Fil: Morbidoni, Héctor Ricardo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina |
| Databáze: | OpenAIRE |
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