MicroRNA-4497 functions as a tumor suppressor in laryngeal squamous cell carcinoma via negatively modulation the GBX2
| Autor: | Litao Zhang, Shi-Xiong Tang, Xudong Chen |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
MAPK/ERK pathway
Cell Survival MAP Kinase Signaling System Blotting Western Colony Count Microbial bcl-X Protein Apoptosis medicine.disease_cause Real-Time Polymerase Chain Reaction Flow cytometry 03 medical and health sciences 0302 clinical medicine Western blot Cell Line Tumor microRNA Medicine Humans MTT assay Viability assay RNA Messenger 030223 otorhinolaryngology Laryngeal Neoplasms Cell Proliferation Homeodomain Proteins medicine.diagnostic_test business.industry Squamous Cell Carcinoma of Head and Neck General Medicine Flow Cytometry MicroRNAs Otorhinolaryngology Proto-Oncogene Proteins c-bcl-2 030220 oncology & carcinogenesis Cancer research Myeloid Cell Leukemia Sequence 1 Protein Surgery business Carcinogenesis |
| Zdroj: | Auris, nasus, larynx. 46(1) |
| ISSN: | 1879-1476 |
| Popis: | Objective MicroRNAs (miRNAs) are aberrantly expressed in various tumors and play a critical role in the progression and development of tumors. However, there is little information about the role of miR-4497 in laryngeal squamous cell carcinoma (LSCC). The aim of this study is to investigate the role of miR-4497 in LSCC. Methods MiR-4497 expression in tumor tissues and adjacent normal tissues was measured by RT-PCR. The effects of miR-4497 on cell viability and apoptosis were evaluated by the MTT assay, Flow cytometry and caspase-3 activity assay. Western blot analysis was used to measure the expression of various proteins. Bioinformatic analysis and luciferase reporter assay were applied to investigate the relationship between miR-4497 and GBX2. Results We found that miR-4497 expression was downregulated in LSCC tumor tissues and cell lines compared to the normal counterparts. Overexpression of miR-4497 inhibits the proliferation and induces apoptosis of LSCC cells accompanied by the down-regulation of anti-apoptotic Bcl-2 proteins. Mechanisms investigation revealed that GBX2 is a direct target of miR-4497. miR-4497 expression was inversely correlated with GBX2 expression in LSCC tissues. Moreover, overexpression of miR-4497 leads to the activation of ERK, JNK but not p38. Inhibition of ERK by specific inhibitor SCH772984 could interfere the apoptosis induced by overexpression of miR-4497. Conclusion Therefore, our results indicate that miR-4497 may play a suppressive role in LSCC by targeting GBX2, which offer new insights into the tumorigenesis of LSCC. |
| Databáze: | OpenAIRE |
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