Age dependent nitro-oxidative load and melatonin receptor expression in the spleen and immunity of goat Capra hircus
| Autor: | Amaresh Kumar Singh, Chandana Haldar |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Aging
medicine.medical_specialty Receptor expression Receptors Melatonin Nitric Oxide Synthase Type II Spleen Lymphocyte proliferation Biology medicine.disease_cause Biochemistry Melatonin receptor Melatonin Endocrinology Immune system Internal medicine Genetics medicine Animals Molecular Biology bcl-2-Associated X Protein Immunity Cellular Caspase 3 Receptor Melatonin MT2 Goats Receptor Melatonin MT1 Cell Biology Immunosenescence Reactive Nitrogen Species medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Oxidative stress medicine.drug |
| Zdroj: | Experimental Gerontology. 60:72-78 |
| ISSN: | 0531-5565 |
| DOI: | 10.1016/j.exger.2014.09.017 |
| Popis: | The decline in the plasma level of melatonin has been associated with increased oxidative stress in the physiological system while aging. The increased levels of oxidants are known to augment the nitro-oxidative stress, which induces the apoptotic factors in lymphoid organs leading to age dependent immunosenescence. There are no reports to date that can suggest how the age dependent nitro-oxidative stress can influence the melatonin membrane MT1/MT2R expression and immune status of any small ruminant. In the present study, we noted the expression of melatonin receptors MT1R and MT2R and inducible nitric oxide synthase (iNOS) along with the apoptotic markers (viz. Bcl-2, Bax and Pro-caspase-3) in the spleen of young, middle-aged and old-aged Indian goat Capra hircus. The lymphocyte proliferation was also recorded along with the total nitrite and nitrate ion concentration (NOx) in the spleen and plasma. An age dependent decline in MT1R and MT2R expressions and lymphocyte proliferation with increased level of reactive nitrogen species (RNS) and iNOS expression was noted. An increased Bax/Bcl-2 ratio and a decreased Pro-caspase-3 expression were observed in the spleen of goat with an age dependent decline in the peripheral melatonin level. This decline in melatonin along with reduced melatonin receptor (MT1/MT2) expression and elevated RNS level in the spleen with aging might have an important role in the regulation of immune function of goats. Our observations suggest that the age-associated immunosenescence observed in goats can be a consequence of declining melatonin and its receptor expression and induction of apoptotic factors influenced by the increased RNS level that deteriorates the proper functioning of the spleen. |
| Databáze: | OpenAIRE |
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