Studies on the role of red blood cell glycoproteins as receptors for invasion by Plasmodium falciparum merozoites
| Autor: | J D Haynes, M H McGinniss, Louis H. Miller, Russell J. Howard |
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| Rok vydání: | 1982 |
| Předmět: |
Erythrocytes
Pan troglodytes Plasmodium falciparum Acetylglucosamine Structure-Activity Relationship medicine Animals Glycophorin Glycophorins Receptors Immunologic Receptor Molecular Biology Glycoproteins chemistry.chemical_classification biology GYPB Erythrocyte Membrane biology.organism_classification Molecular biology In vitro Red blood cell medicine.anatomical_structure Carbohydrate Sequence chemistry Biochemistry biology.protein MNSs Blood-Group System Parasitology Glycoprotein Neuraminidase |
| Zdroj: | Molecular and Biochemical Parasitology. 6:303-315 |
| ISSN: | 0166-6851 |
| Popis: | The mechanism of invasion of human red blood cells by Plasmodium falciparum merozoites has been studied by several indirect methods. Red blood cells of the S+s+U+ and S-s-U- blood group phenotypes were trypsin treated and their susceptibility to invasion measured. Trypsin-treated S+s+U+ cells lack the portion of glycophorin A which bears the MN blood group determinants but possess glycophorin B, whereas trypsin-treated S-s-U- cells lack both the glycophorin A MN determinants and the glycophorin B molecule. Since the treated S-s-U- cells showed an even greater loss in susceptibility to invasion that the treated S+s+U+ cells, we conclude that glycophorin B does have a role In merozoite recognition, although it appears less important than glycophorin A. Attempts to decrease invasion by pretreatment with glycosidases were unsuccessful, except for the previously reported effect of neuraminidase. N-acetyl-D-glucosamine decreases the appearance of ring-stage parasites after in vitro reinvasion of P. falciparum. However, the persistence of intact and lysed schizont-infected cells when N-acetyl-D-glucosamine was present, several hours after disappearance of these cells from control cultures, leads us to conclude that this sugar has a deleterious effect on terminal stages of parasite maturation. It is therefore not possible to conclude that N-acetyl-D-glucosamine inhibits merozoite attachment and reinvasion specifically by competition for the receptor. |
| Databáze: | OpenAIRE |
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