Self-sampling for human papillomavirus DNA detection : a preliminary study of compliance and feasibility in BOLIVIA
| Autor: | Véronique Fontaine, Marc Baay, Leyddy Cáceres, Rosse Mary Yañez, Diego Bellot, Ligia Villarroel, Pedro Surriabre, Pamela Calle, Andrea Torrico, Gustavo Allende, Patricia Rodriguez, Jaime Barriga, Shirley Rojas, Marcela Prado, Karina Ustariz |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Adult
medicine.medical_specialty Bolivia Human papillomavirus Uterine Cervical Neoplasms Hpv detection lcsh:Gynecology and obstetrics law.invention Specimen Handling 03 medical and health sciences Diagnostic Self Evaluation 0302 clinical medicine Acceptability law Immunologie Glass slide Devices Medicine Humans 030212 general & internal medicine Human papillomavirus DNA detection lcsh:RG1-991 Early Detection of Cancer Kappa value business.industry Obstetrics lcsh:Public aspects of medicine Papillomavirus Infections Biologie moléculaire Obstetrics and Gynecology HPV screening lcsh:RA1-1270 Self-sampling General Medicine Cervical cells Middle Aged Reproductive Medicine 030220 oncology & carcinogenesis Biologie cellulaire Cotton swab Female Human medicine Microbiologie et protistologie [bacteriol.virolog.mycolog.] business Self sampling Research Article |
| Zdroj: | BMC women's health BMC Women's Health BMC Women's Health, Vol 17, Iss 1, Pp 1-7 (2017) BMC women's health, 17 (1 |
| ISSN: | 1472-6874 |
| Popis: | Background: Cervical cancer incidence and mortality rates in Bolivia are among the highest in Latin America. This investigation aims to evaluate the possibility of using simple devices, e.g. a cotton swab and a glass slide, for self-sampling in order to detect human papillomavirus (HPV) DNA by PCR in cervico-vaginal cells. Methods: In the first phase of our study we evaluated the use of a glass slide as a transport medium for cervical cells. A physician took paired-cervical samples from 235 women. One sample was transported in Easyfix® solution and the other sample was smeared over a glass slide. Both were further analyzed and compared for human DNA recovery and HPV detection. A kappa value was determined to evaluate the agreement between the HPV DNA detection rates. In the second phase of the study, 222 women from the urban, peri-urban and rural regions of Cochabamba were requested to perform self-sampling using the following devices: a cotton swab combined with a glass slide, and a vaginal tampon. Women gave their opinion about the self-sampling technique. Finally, the agreement for high risk-HPV detection between self- and physician-collected samples was performed in 201 samples in order to evaluate the self-sampling technique. Results: Firstly, the comparison between Easyfix® solution and the glass slide to transport clinical samples gave a good agreement for HPV DNA detection (Κ = 0.71, 95% CI 0.60-0.81). Secondly, self-sampling, especially with cotton swab combined with glass slide, would generally be preferred over clinician sampling for a screening program based on HPV detection. Finally, we showed a good agreement between self- and physician collected samples for high risk-HPV detection (Κ = 0.71, 95% CI 0.55-0.88). Conclusions: Simple devices such as a cotton swab and a glass slide can be used to perform self-sampling and HPV DNA detection. Furthermore, most Bolivian women preferred self-sampling over clinician-sampling for cervical cancer screening. SCOPUS: ar.j info:eu-repo/semantics/published |
| Databáze: | OpenAIRE |
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