Endocytosis of nutrient transporters in fungi: the ART of connecting signaling and trafficking
| Autor: | Gabriel Talaia, Margarida Casal, Hernâni Gerós, Sandra Paiva, Rosana Maria Abreu Alves, Cláudia Barata-Antunes, Robert Mans |
|---|---|
| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
EMCs
eisosome membrane compartments Signaling pathways Ubiquitylation AP adaptor protein Arrestins Endocytic cycle Fe iron PVE prevacuolar endosome Review Biochemistry VPS vacuolar protein sorting IF inward-facing APC amino acid-polyamine-organocation TRY titer rate and yield 0302 clinical medicine MVB multi vesicular bodies Ubiquitin Structural Biology Met methionine Mn manganese MCCs/eisosomes Ub ubiquitin Cu copper Zn Zinc Cell factories Glu glutamic acid 0303 health sciences Arts arrestin‐related trafficking adaptors biology Chemistry C-terminus carboxyl-terminus NCS2 nucleobase cation symporter family 2 Endocytosis Computer Science Applications Ubiquitin ligase Cell biology fAATs fungal AA transporters Nutrient transporters ACT amino Acid/Choline Transporter 030220 oncology & carcinogenesis PM plasma membrane NAT nucleobase Ascorbate Transporter Signal transduction ESCRT endosomal sorting complex required for transport Lys lysine TMSs transmembrane segments TORC1 target of rapamycin complex 1 Biotechnology N-terminus amino-terminus Conformational changes Trp tryptophan PEST proline (P) glutamic acid (E) serine (S) and threonine (T) Saccharomyces cerevisiae Biophysics OF outward-facing Asp aspartic acid LID loop Interaction Domain ER endoplasmic reticulum H+ proton 03 medical and health sciences Genetics NH4+ ammonium GAAC general amino acid control 030304 developmental biology ComputingMethodologies_COMPUTERGRAPHICS Arg arginine Science & Technology Structure-function TGN trans-Golgi network Fungi C carbon MFS major facilitator superfamily Transporter Tyr tyrosine biology.organism_classification LAT L-type Amino acid Transporter Cytosol MCPs membrane compartments of Pma1 AAs amino acids Metabolism NCS1 nucleobase/Cation Symporter 1 biology.protein YAT yeast Amino acid Transporter Aspergilli MCCs membrane compartments containing the arginine permease Can1 W/V weight per volume Endocytic signals DUBs deubiquitinating enzymes N nitrogen TP248.13-248.65 |
| Zdroj: | Computational and Structural Biotechnology Journal, 19 Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Computational and Structural Biotechnology Journal Computational and Structural Biotechnology Journal, Vol 19, Iss, Pp 1713-1737 (2021) |
| ISSN: | 2001-0370 |
| Popis: | Plasma membrane transporters play pivotal roles in the import of nutrients, including sugars, amino acids, nucleobases, carboxylic acids, and metal ions, that surround fungal cells. The selective removal of these transporters by endocytosis is one of the most important regulatory mechanisms that ensures a rapid adaptation of cells to the changing environment (e.g., nutrient fluctuations or different stresses). At the heart of this mechanism lies a network of proteins that include the arrestin?related trafficking adaptors (ARTs) which link the ubiquitin ligase Rsp5 to nutrient transporters and endocytic factors. Transporter conformational changes, as well as dynamic interactions between its cytosolic termini/loops and with lipids of the plasma membrane, are also critical during the endocytic process. Here, we review the current knowledge and recent findings on the molecular mechanisms involved in nutrient transporter endocytosis, both in the budding yeast Saccharomyces cerevisiae and in some species of the filamentous fungus Aspergillus. We elaborate on the physiological importance of tightly regulated endocytosis for cellular fitness under dynamic conditions found in nature and highlight how further understanding and engineering of this process is essential to maximize titer, rate and yield (TRY)-values of engineered cell factories in industrial biotechnological processes. We are grateful to Dr. George Diallinas and Dr. Christos Gournas for their suggestions and critical reading of this manuscript. We thank Dr. Jack Pronk for his fruitful suggestions. Work at CBMA was supported by the ‘‘Contrato-Programa” UIDB/04050/2020 funded by Portuguese national funds through the FCT I.P. BA and RA acknowledge FCT for the PD/BD/135208/2017 and PD/BD/113813/2015 PhD grants, respectively. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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