Control of human thymocyte migration by Neuropilin-1/Semaphorin-3A-mediated interactions
| Autor: | Yves Lepelletier, Mireille Dardenne, Ana Cristina Martins de Almeida Nogueira, Olivier Hermine, Wilson Savino, Salete Smaniotto, Déa Maria Serra Villa-Verde, Réda Hadj-Slimane |
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| Přispěvatelé: | Cytokines, hématopoïèse et réponse immune (CHRI), Université Paris Descartes - Paris 5 (UPD5) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Centre National de la Recherche Scientifique (CNRS), Department of Immunology (FONDATION OSWALDO CRUZ), Fundação Oswaldo Cruz (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP) - Fundação Oswaldo Cruz (FIOCRUZ) - Réseau International des Instituts Pasteur (RIIP) - Fundação Oswaldo Cruz (FIOCRUZ), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Universidade Federal de Alagoas = Federal University of Alagoas (UFAL), Pharmacochimie Moléculaire et Cellulaire (PMC - UMR_S 648), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5), Tragex Pharma, This work was developed in the Institut National de la Santé et de la Recherche Médicale (INSERM)/Fundação Oswaldo Cruz (FIOCRUZ) Associated Laboratory of Immunology. Y.L. is a recipient of a Ligue National contre le Cancer grant. This work was supported by grants from INSERM/FIOCRUZ and Comissão de Aperfeiçoamento de Pessoal de Nível Superior/Comité Français d'Évaluation de la Coopération Universitaire avec le Brésil conjoint French–Brazilian programs, Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (Brazil), Les Programmes Internationaux de Coopération Scientifique Centre National de la Recherche Scientifique (France), and Ligue National contre le Cancer (France)., We thank the cardiac chirurgical pediatric offices (Necker Hospital, Paris, France) and Laranjeiras Hospital in Rio de Janeiro, Laboratory on Thymus Research - Department of Immunology / Laboratório de Pesquisas sobre o Timo - Departamento de Imunologia [Rio de Janeiro], Instituto Oswaldo Cruz / Oswaldo Cruz Institute [Rio de Janeiro] (IOC), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Fundação Oswaldo Cruz (FIOCRUZ) |
| Rok vydání: | 2007 |
| Předmět: |
MESH: Chemotaxis
Integrins Ligands 0302 clinical medicine Cell Movement Neuropilin 1 MESH: Receptors Antigen T-Cell/metabolism MESH: Up-Regulation MESH: Ligands MESH: Extracellular Matrix/metabolism 0303 health sciences Multidisciplinary Chemotaxis MESH: Gene Expression Regulation MESH: Thymus Gland/metabolism Biological Sciences MESH: Semaphorin-3A/metabolism MESH: Integrins/metabolism Extracellular Matrix Up-Regulation Cell biology Thymocyte medicine.anatomical_structure 030220 oncology & carcinogenesis MESH: Neuropilin-1/metabolism Thymocyte migration Protein Binding MESH: Cell Movement animal structures T cell education Receptors Antigen T-Cell [SDV.CAN]Life Sciences [q-bio]/Cancer Thymus Gland Biology MESH: Cell Adhesion 03 medical and health sciences Chemorepulsion MESH: Interleukin-7/metabolism Cell Adhesion medicine MESH: Protein Binding Humans [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Cell adhesion 030304 developmental biology MESH: Humans Interleukin-7 T-cell receptor Semaphorin-3A Dendritic cell thymocyte adhesion and migration MESH: Thymus Gland/cytology Neuropilin-1 Gene Expression Regulation |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2007, 104, pp.5545-5550 Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2007, 104, pp.5545-5550. ⟨10.1073/pnas.0700705104⟩ |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.0700705104 |
| Popis: | It is largely established that molecules first discovered in the nervous system are also found in the immune system. Neuropilin-1 (NP-1) was initially identified to mediate semaphorin-induced chemorepulsion during brain development and is also involved in peripheral T cell/dendritic cell interactions. Herein, we studied NP-1 during T cell development in the human thymus. NP-1 is expressed in both cortex and medulla of thymic lobules, being found in distinct CD4/CD8-defined thymocyte subsets. NP-1 is also found in thymic epithelial cells (TEC) in situ and in vitro , and is recruited at the site of TEC–thymocyte contact. Moreover, NP-1 was rapidly up-regulated during thymocyte stimulation by T cell receptor (TCR) and IL-7 or after adhesion to TEC. Semaphorin-3A (Sema-3A), a natural ligand of NP-1, is also present in human thymus, both in TEC and thymocytes, being up-regulated in thymocytes after TCR engagement. Functionally, Sema-3A decreases the adhesion capacity of NP-1 + thymocytes and induces their migration by a repulsive effect. In conclusion, we show here that NP-1/Sema-3A-mediated interactions participate in the control of human thymocyte development. |
| Databáze: | OpenAIRE |
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