Multimodal imaging Gd-nanoparticles functionalized with Pittsburgh compound B or a nanobody for amyloid plaques targeting

Autor: C. Marquette, François Lux, Eric Allémann, Éva Tóth, Mireille Dumoulin, Laurence Heinrich-Balard, Olivier Tillement, Pascaline Rivory, Maria João Saraiva, Jean-François Morfin, Jonathan Pansieri, Nathalie Martine Stransky-Heilkron, Marie Plissonneau, Vincent Forge
Přispěvatelé: Laboratoire de Chimie et Biologie des Métaux (LCBM - UMR 5249), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Nano-H S.A.S, Nano-H S.A.S, 38070 Saint Quentin Fallavier, France, Institut Lumière Matière [Villeurbanne] (ILM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), School of Pharmaceutical Sciences, Université de Genève = University of Geneva (UNIGE), Laboratoire d'Enzymologie et Repliement des Protéines, Centre d'Ingénierie des Protéines, Université de Liège, Matériaux, ingénierie et science [Villeurbanne] (MATEIS), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Instituto de Inovação e Investigação em Saúde (I3S), University of Porto, Portugal, Molecular Neurobiology Group, IBMC - Institute for Molecular & Cell Biology, University of Porto, 4150-180 Porto, Portugal., GON, Nathalie, Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), University of Geneva [Switzerland], Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)
Rok vydání: 2017
Předmět:
0301 basic medicine
[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging
Medicine (miscellaneous)
Nanoparticle
Amylin
Peptide
Gadolinium
Plaque
Amyloid
Multimodal Imaging
chemistry.chemical_compound
Amyloid disease
Mice
General Materials Science
chemistry.chemical_classification
ddc:615
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology
Aniline Compounds
biology
Brain
Immunohistochemistry
Islet Amyloid Polypeptide
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biophysics
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
amyloid imaging
Materials science
Amyloid
Biomedical Engineering
[SDV.BBM.BP] Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biophysics
Bioengineering
Development
03 medical and health sciences
Alzheimer Disease
Animals
Humans
[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Amyloid beta-Peptides
PIB
amyloidoses
gadolinium-based nanoparticles
Single-Domain Antibodies
Combinatorial chemistry
In vitro
nanobody
Transthyretin
Thiazoles
[SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging
030104 developmental biology
chemistry
Diabetes Mellitus
Type 2
biology.protein
Biophysics
Nanoparticles
Pittsburgh compound B
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Zdroj: Nanomedicine
Nanomedicine, 2017, 12 (14), pp.1675-1687. ⟨10.2217/nnm-2017-0079⟩
Nanomedicine, Future Medicine, 2017, 12 (14), pp.1675-1687. ⟨10.2217/nnm-2017-0079⟩
Nanomedicine, Vol. 12, No 14 (2017) pp. 1675-1687
ISSN: 1748-6963
1743-5889
1549-9634
DOI: 10.2217/nnm-2017-0079⟩
Popis: Aim: Gadolinium-based nanoparticles were functionalized with either the Pittsburgh compound B or a nanobody (B10AP) in order to create multimodal tools for an early diagnosis of amyloidoses. Materials & methods: The ability of the functionalized nanoparticles to target amyloid fibrils made of β-amyloid peptide, amylin or Val30Met-mutated transthyretin formed in vitro or from pathological tissues was investigated by a range of spectroscopic and biophysics techniques including fluorescence microscopy. Results: Nanoparticles functionalized by both probes efficiently interacted with the three types of amyloid fibrils, with KD values in 10 micromolar and 10 nanomolar range for, respectively, Pittsburgh compound B and B10AP nanoparticles. Moreover, they allowed the detection of amyloid deposits on pathological tissues. Conclusion: Such functionalized nanoparticles could represent promising flexible and multimodal imaging tools for the early diagnostic of amyloid diseases, in other words, Alzheimer's disease, Type 2 diabetes mellitus and the familial amyloidotic polyneuropathy.
Databáze: OpenAIRE
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