Preclinical validation of salivary biomarkers for primary Sjögren's syndrome
| Autor: | Hui Zhou, Martha Arellano-Garcia, Lei Zhang, Arjan Vissink, Kai Gao, David Elashoff, David T.W. Wong, Shen Hu, Rodney P. E. Pollard, Cees G. M. Kallenberg |
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| Přispěvatelé: | Faculteit Medische Wetenschappen/UMCG, Personalized Healthcare Technology (PHT), Translational Immunology Groningen (TRIGR) |
| Rok vydání: | 2009 |
| Předmět: |
Adult
Systemic disease medicine.medical_specialty Myeloid Cathepsin D CLASSIFICATION Article Cohort Studies DOUBLE-BLIND Rheumatology Immunopathology Internal medicine medicine CRITERIA Humans RNA Messenger Saliva Autoimmune disease Beta-2 microglobulin business.industry MNDA RITUXIMAB TREATMENT Middle Aged medicine.disease EFFICACY medicine.anatomical_structure Real-time polymerase chain reaction Sjogren's Syndrome Phosphopyruvate Hydratase Immunology Female business beta 2-Microglobulin Biomarkers |
| Zdroj: | ARTHRITIS CARE & RESEARCH, 62(11), 1633-1638. Wiley |
| ISSN: | 2151-4658 2151-464X |
| Popis: | Objective. Sjogren's syndrome (SS) is a systemic autoimmune disease with a variety of presenting symptoms that may delay its diagnosis. We previously discovered a number of candidate salivary biomarkers for primary SS using both mass spectrometry and expression microarray analysis. In the current study, we aimed to verify these candidate biomarkers in independent patient populations and to evaluate their predictive values for primary SS detection.Methods. In total, 34 patients with primary SS, 34 patients with systemic lupus erythematosus (SLE), and 34 healthy individuals were enrolled for the validation studies. Salivary protein biomarkers were measured using either Western blotting or enzyme-linked immunosorbent assay, and the messenger RNA (mRNA) biomarkers were measured using quantitative polymerase chain reaction. Statistical analysis was performed using R software, version 2.9.Results. Three protein biomarkers (cathepsin D [CPD], alpha-enolase, and beta(2)-microglobulin [beta(2)m]) and 3 mRNA biomarkers (myeloid cell nuclear differentiation antigen [MNDA], guanylate binding protein 2 [GBP-2], and low-affinity IIIb receptor for the Fc fragment of IgG) were significantly elevated in patients with primary SS compared with both SLE patients and healthy controls. The combination of 3 protein biomarkers, CPD, alpha-enolase, and beta(2)m, yielded a receiver operating characteristic (ROC) value of 0.99 in distinguishing primary SS from healthy controls. The combination of protein biomarkers beta(2)m and 2 mRNA biomarkers, MNDA and GBP-2, reached an ROC of 0.95 in discriminating primary SS from SLE.Conclusion. We have successfully verified a panel of protein and mRNA biomarkers that can discriminate primary SS from both SLE and healthy controls. If further validated in patients with primary SS and those with sicca symptoms but no autoimmune disease, these biomarkers may lead to a simple yet highly discriminatory clinical tool for diagnosis of primary SS. |
| Databáze: | OpenAIRE |
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