Impact of Reck expression and promoter activity in neuronal in vitro differentiation
| Autor: | Mari Cleide Sogayar, Sheila M.B. Winnischofer, Ricardo Cesar Cintra, Joana D'Arc Campeiro, Isis C C Nascimento, Mirian A. F. Hayashi, Juliano R. Guerreiro, Thais Assis-Ribas, Henning Ulrich, Maria Trombetta-Lima |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Tumor suppressor gene Matrix metalloproteinase Kazal Motifs Pheochromocytoma 03 medical and health sciences GLICOPROTEÍNAS 0302 clinical medicine Genetics medicine Reck promoter activity Molecular Biology chemistry.chemical_classification P19 teratocarcinoma biology PC12 pheochromocytoma Wnt signaling pathway General Medicine medicine.disease In vitro Cell biology 030104 developmental biology Tubulin Neuronal differentiation chemistry 030220 oncology & carcinogenesis biology.protein Glycoprotein Reck tumor suppressor gene |
| Zdroj: | Repositório Institucional da UNIFESP Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1573-4978 0301-4851 |
| Popis: | Reck (REversion-inducing Cysteine-rich protein with Kazal motifs) tumor suppressor gene encodes a multifunctional glycoprotein which inhibits the activity of several matrix metalloproteinases (MMPs), and has the ability to modulate the Notch and canonical Wnt pathways. Reck-deficient neuro-progenitor cells undergo precocious differentiation; however, modulation of Reck expression during progression of the neuronal differentiation process is yet to be characterized. In the present study, we demonstrate that Reck expression levels are increased during in vitro neuronal differentiation of PC12 pheochromocytoma cells and P19 murine teratocarcinoma cells and characterize mouse Reck promoter activity during this process. Increased Reck promoter activity was found upon induction of differentiation in PC12 cells, in accordance with its increased mRNA expression levels in mouse in vitro models. Interestingly, Reck overexpression, prior to the beginning of the differentiation protocol, led to diminished efficiency of the neuronal differentiation process. Taken together, our findings suggest that increased Reck expression at early stages of differentiation diminishes the number of neuron-like cells, which are positive for the beta-3 tubulin marker. Our data highlight the importance of Reck expression evaluation to optimize in vitro neuronal differentiation protocols. |
| Databáze: | OpenAIRE |
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