Effect of an osmotic stress on multicellular aggregates
| Autor: | Benjamin Brunel, Antoine Delon, Sylvain Monnier, Monika E. Dolega, Giovanni Cappello, Morgan Delarue |
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| Přispěvatelé: | Physico-Chimie-Curie (PCC), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC), Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] (LIPhy), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Biocompatible polymers Osmotic shock [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] Acrylic Resins Cell Culture Techniques Biology Mechanotransduction Cellular General Biochemistry Genetics and Molecular Biology Stress (mechanics) Mice 03 medical and health sciences Osmotic Pressure Cell Line Tumor Spheroids Cellular Animals Osmotic pressure Molecular Biology Cell Aggregation Cell Size Matrigel Isotropy Cell biology Multicellular organism 030104 developmental biology Multicellular spheroid |
| Zdroj: | Methods Methods, Elsevier, 2015, 94, pp.114-119. ⟨10.1016/j.ymeth.2015.07.009⟩ Methods, 2015, 94, pp.114-119. ⟨10.1016/j.ymeth.2015.07.009⟩ |
| ISSN: | 1046-2023 1095-9130 |
| DOI: | 10.1016/j.ymeth.2015.07.009 |
| Popis: | International audience; There is increasing evidence that multicellular structures respond to mechanical cues, such as the confinement and compression exerted by the surrounding environment. In order to understand the response of tissues to stress, we investigate the effect of an isotropic stress on different biological systems. The stress is generated using the osmotic pressure induced by a biocompatible polymer. We compare the response of multicellular spheroids, individual cells and matrigel to the same osmotic perturbation. Our findings indicate that the osmotic pressure occasioned by polymers acts on these systems like an isotropic mechanical stress. When submitted to this pressure, the volume of multicellular spheroids decreases much more than one could expect from the behavior of individual cells. |
| Databáze: | OpenAIRE |
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