Multiple central WB-4101 binding sites and the selectivity of prazosin
| Autor: | Thomas F. Lyon, William C. Randall |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Stereochemistry
Alpha (ethology) In Vitro Techniques Dioxins Binding Competitive General Biochemistry Genetics and Molecular Biology Clonidine Dioxanes Norepinephrine Prazosin medicine Radioligand Animals General Pharmacology Toxicology and Pharmaceutics Binding site Receptor Adrenergic alpha-Antagonists Binding Sites Membranes Chemistry Brain General Medicine Receptors Adrenergic alpha Lower affinity Kinetics Membrane Quinazolines Cattle Selectivity medicine.drug |
| Zdroj: | Life sciences. 26(14) |
| ISSN: | 0024-3205 |
| Popis: | Under the conditions employed in a standard displacement assay of [3H]WB-4101 from calf neocortical membrane homogenates (0.22 nM total [3H]WB-4101), 70–80% of this radioligand is bound to a high affinity receptor site and 20–30% to a second, lower affinity site. This latter site is not the one previously shown to bind alpha agonists. Binding parameters for WB-4101 to each site have been determined. Prazonin is extremely selective for the high affinity site as indicated by a biphasic displacement of [3H]WB-4101. |
| Databáze: | OpenAIRE |
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