Sophorolipid Butyl Ester Diacetate Does Not Affect Macrophage Polarization but Enhances Astrocytic Glial Fibrillary Acidic Protein Expression at Micromolar Concentrations in Vitro
| Autor: | Alexis M. Ziemba, Ryan J. Gilbert, Richard A. Gross, Cheryl M. Hanes, Filbert Totsingan, Manoj K. Gottipati, Michelle R. Lennartz |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Physiology Cell Survival Cognitive Neuroscience Macrophage polarization Biochemistry Article 03 medical and health sciences Mice 0302 clinical medicine Glycolipid Glial Fibrillary Acidic Protein medicine Macrophage Animals Glial fibrillary acidic protein biology Chemistry Sophorolipid Macrophages Cell Polarity Cell Biology General Medicine Phenotype In vitro Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Neuroprotective Agents Astrocytes biology.protein Glycolipids 030217 neurology & neurosurgery Astrocyte |
| Popis: | Peritoneal macrophages (PMACs) and spinal cord astrocytes were exposed to varying concentrations of soluble sophorolipid butyl ester diacetate (SLBEDA) in vitro. Macrophages and astrocytes demonstrated no decrease in viability in response to SLBEDA. Studying pro- and anti-inflammatory genes, PMACs did not show a shift toward a pro-inflammatory phenotype. However, at higher concentrations (3 and 30 μM), astrocytes showed an increase in their expression of glial acidic fibrillary protein. This novel category of compounds poses low risk to PMAC and astrocyte viability; however, the effect on PMAC polarization and astrocyte reactivity requires more elucidation. |
| Databáze: | OpenAIRE |
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