RNA sequencing analysis of salt tolerance in soybean (Glycine max)
| Autor: | Chengjun Wu, Kenneth L. Korth, Pengyin Chen, Julie Thomas, Andy Pereira, Subodh K. Srivastava, Ailan Zeng, Kristofor R. Brye, Jianxin Ma, Jieqing Ping, Floyd Hancock |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Genetics Germplasm 0303 health sciences education.field_of_study Chlorosis Genotype Population RNA Salt Tolerance Biology 01 natural sciences Fold change 03 medical and health sciences Gene Expression Regulation Plant Gene expression Soybeans Transcriptome education Gene 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Genomics. 111:629-635 |
| ISSN: | 0888-7543 |
| DOI: | 10.1016/j.ygeno.2018.03.020 |
| Popis: | Salt stress causes foliar chlorosis and scorch, plant stunting, and eventually yield reduction in soybean. There are differential responses, namely tolerance (excluder) and intolerance (includer), among soybean germplasm. However, the genetic and physiological mechanisms for salt tolerance is complex and not clear yet. Based on the results from the screening of the RA-452 x Osage mapping population, two F4:6 lines with extreme responses, most tolerant and most sensitive, were selected for a time-course gene expression study in which the 250 mM NaCl treatment was initially imposed at the V1 stage and continued for 24 h (hrs). Total RNA was isolated from the leaves harvested at 0, 6, 12, 24 h after the initiation of salt treatment, respectively. The RNA-Seq analysis was conducted to compare the salt tolerant genotype with salt sensitive genotype at each time point using RNA-Seq pipeline method. A total of 2374, 998, 1746, and 630 differentially expressed genes (DEGs) between salt-tolerant line and salt-sensitive line, were found at 0, 6, 12, and 24 h, respectively. The expression patterns of 154 common DEGs among all the time points were investigated, of which, six common DEGs were upregulated and seven common DEGs were downregulated in salt-tolerant line. Moreover, 13 common DEGs were dramatically expressed at all the time points. Based on Log2 (fold change) of expression level of salt-tolerant line to salt-sensitive line and gene annotation, Glyma.02G228100, Glyma.03G226000, Glyma.03G031000, Glyma.03G031400, Glyma.04G180300, Glyma.04G180400, Glyma.05 g204600, Glyma.08G189600, Glyma.13G042200, and Glyma.17G173200, were considered to be the key potential genes involving in the salt-tolerance mechanism in the soybean salt-tolerant line. |
| Databáze: | OpenAIRE |
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