Viruses with different genome types adopt a similar strategy to pack nucleic acids based on positively charged protein domains
| Autor: | Rodrigo D. Requião, Marcelo Ribeiro-Alves, Tatiana Domitrovic, Fernando L. Palhano, Mariana H. Moreira, Rodolfo L. Carneiro, Silvana Rossetto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Icosahedral symmetry viruses Static Electricity Protein domain Lysine lcsh:Medicine Genome Viral Genome Article Phosphates Viral Proteins 03 medical and health sciences Protein Domains Virology Nucleic Acids Nucleotide Amino Acid Sequence lcsh:Science chemistry.chemical_classification Genetics Multidisciplinary 030102 biochemistry & molecular biology Nucleotides Virus Assembly lcsh:R Virion Computational biology and bioinformatics Amino acid 030104 developmental biology chemistry Capsid Viruses Nucleic acid Capsid Proteins lcsh:Q |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-62328-w |
| Popis: | Capsid proteins often present a positively charged arginine-rich sequence at their terminal regions, which has a fundamental role in genome packaging and particle stability for some icosahedral viruses. These sequences show little to no conservation and are structurally dynamic such that they cannot be easily detected by common sequence or structure comparisons. As a result, the occurrence and distribution of positively charged domains across the viral universe are unknown. Based on the net charge calculation of discrete protein segments, we identified proteins containing amino acid stretches with a notably high net charge (Q > + 17), which are enriched in icosahedral viruses with a distinctive bias towards arginine over lysine. We used viral particle structural data to calculate the total electrostatic charge derived from the most positively charged protein segment of capsid proteins and correlated these values with genome charges arising from the phosphates of each nucleotide. We obtained a positive correlation (r = 0.91, p-value |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |