Immunophenotypes of Chronic Myelomonocytic Leukemia (CMML) Subtypes by Flow Cytometry: A Comparison of CMML-1 vs CMML-2, Myeloproliferative vs Dysplastic, De Novo vs Therapy-Related, and CMML-Specific Cytogenetic Risk Subtypes
| Autor: | Steven H. Kroft, Atousa Ordobazari, Alexandra M. Harrington, Paul Hosking, Lindsay A. Schelling, Horatiu Olteanu |
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| Rok vydání: | 2016 |
| Předmět: |
Male
medicine.medical_specialty CD14 Chronic myelomonocytic leukemia CD15 Granulocyte Biology Gastroenterology Immunophenotyping 03 medical and health sciences 0302 clinical medicine Antigen Antigens CD Risk Factors hemic and lymphatic diseases Internal medicine medicine Biomarkers Tumor Humans Aged Aged 80 and over Cytogenetics Leukemia Myelomonocytic Chronic General Medicine Middle Aged medicine.disease Flow Cytometry medicine.anatomical_structure 030220 oncology & carcinogenesis Immunology Female Bone marrow 030215 immunology |
| Zdroj: | American journal of clinical pathology. 146(2) |
| ISSN: | 1943-7722 |
| Popis: | Objectives: We sought to immunophenotype blasts, monocytes, and granulocytes in chronic myelomonocytic leukemias (CMMLs) and compare CMML subtypes, to identify if significant antigen expression differences existed. Methods: Bone marrow blasts, monocytes, and granulocytes from CMML subgroups (n = 30; World Health Organization types 1/2, proliferative/dysplastic, therapy related/de novo, and low/intermediate/high cytogenetic risk) were immunophenotypically compared by flow cytometry with 10 nonneoplastic control marrows. Results: Aberrancies were present in blasts of 26 (87%) of 30 CMMLs (26 diagnostic; four follow-up) and six (60%) of 10 controls ( P = .089), monocytes of 28 (93%) of 30 CMMLs and six (60%) of 10 controls ( P = .026), and granulocytes of eight (28%) of 29 CMMLs and zero of 10 controls ( P = .166). Underexpression of CD14 and CD15 on monocytes was more common in CMMLs compared with controls ( P = .008 and P = .043). Statistical analysis showed no significant difference in antigen expression between the CMML subgroups on blasts or monocytes; granulocytes demonstrated more common HLA-DR expression in CMML-2 vs CMML-1. Conclusions: These findings confirm heterogeneity within CMML subgroups and find no specific qualitative or quantitative findings characteristic of a subgroup. |
| Databáze: | OpenAIRE |
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