The Potential of Asiatic Acid in the Reversion of Cyclophosphamide-Induced Hemorrhagic Cystitis in Rats
Autor: | Piotr Radziszewski, Jacek Sienko, Ewa Poleszak, Tomasz Kluz, Daniela Gold, Kajetan Juszczak, Łukasz Zapała, Klaudia Stangel-Wójcikiewicz, Artur Rogowski, Andrzej Wróbel, Marcin Misiek |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Detrusor muscle
Cyclophosphamide QH301-705.5 Hemorrhage Calcitonin gene-related peptide Pharmacology urologic and male genital diseases Catalysis Article Bladder Urothelium Inorganic Chemistry chemistry.chemical_compound Cystitis medicine Animals Physical and Theoretical Chemistry Urothelium Biology (General) Molecular Biology Antineoplastic Agents Alkylating QD1-999 Spectroscopy asiatic acid medicine.diagnostic_test business.industry Organic Chemistry Cystometry General Medicine Malondialdehyde medicine.disease female genital diseases and pregnancy complications Computer Science Applications Rats Disease Models Animal Chemistry medicine.anatomical_structure chemistry cyclophosphamide hemorrhagic cystitis business Pentacyclic Triterpenes Biomarkers Hemorrhagic cystitis medicine.drug |
Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 5853, p 5853 (2021) International Journal of Molecular Sciences Volume 22 Issue 11 |
ISSN: | 1661-6596 1422-0067 |
Popis: | The purpose of this study was to determine if asiatic acid may act efficiently in the model of cyclophosphamide (CYP)-induced cystitis in rats. We performed experiments after administration of CYP (single dose 200 mg/kg, intraperitoneally), asiatic acid (30 mg/kg/day for 14 consecutive days, by oral gavage), or CYP plus asiatic acid, during which conscious cystometry, measurements of urothelium thickness and bladder edema, as well as selected biomarkers analyses were conducted. In rats that received asiatic acid together with CYP, a drop in bladder basal pressure, detrusor overactivity index, non-voiding contraction amplitude, non-voiding contraction frequency, and the area under the pressure curve were observed, when compared to the CYP group. Furthermore, a significant increase in threshold pressure, voided volume, intercontraction interval, bladder compliance, and volume threshold to elicit NVC were found in that group accordingly. Administration of the asiatic acid successfully restored concentrations of biomarkers both in bladder urothelium (BDNF, CGRP, OCT-3, IL-1β, IL-6, NGF, nitrotyrosine, malondialdehyde, TNF-α, SV2A, SNAP23, SNAP25, PAC-1, ORM1, occludin, IGFBP-3, HB-EGF, T–H protein, Z01, and HPX) and detrusor muscle (Rho kinase and VAChT) in CYP-treated rats. Finally, asiatic acid significantly decreased urothelium thickness and bladder oedema. Asiatic acid proved to be a potent and effective drug in the rat model of CYP-induced cystitis. |
Databáze: | OpenAIRE |
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