Spatiotemporal expression of SKIP after rat sciatic nerve crush
| Autor: | Hao Wu, Xiaolong Deng, Xinghai Cheng, Jiao Yang, Yajuan Wu, Haixiang Wei, Hailei Chen, Dong Lou, Hai Wen, Long Long, Youhua Wang |
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| Rok vydání: | 2012 |
| Předmět: |
Male
congenital hereditary and neonatal diseases and abnormalities Polyadenylation Nerve Crush Biochemistry Lesion Rats Sprague-Dawley Cellular and Molecular Neuroscience Western blot Proliferating Cell Nuclear Antigen medicine Animals Cell Proliferation biology medicine.diagnostic_test General Medicine Anatomy Sciatic Nerve Cell biology Proliferating cell nuclear antigen Nerve Regeneration Rats medicine.anatomical_structure nervous system Peripheral nervous system RNA splicing biology.protein Sciatic nerve Schwann Cells medicine.symptom Transforming growth factor Transcription Factors |
| Zdroj: | Neurochemical research. 38(4) |
| ISSN: | 1573-6903 |
| Popis: | Ski-interacting protein (SKIP) is a highly conserved protein from yeast to Human. As an essential spliceosomal component and transcriptional co-regulator it plays an important role in preinitiation, splicing and polyadenylation. SKIP can also combine with Ski to overcome the G1 arrest and the growth-suppressive activities of pRb. Furthermore SKIP has the capacity to augment TGF-β dependent transcription. While the distribution and function of SKIP in peripheral nervous system lesion and regeneration remain unclear. Here, we investigated the spatiotemporal expression of SKIP in an acute sciatic nerve crush model in adult rats. Western Blot analysis revealed that SKIP was expressed in normal sciatic nerves. It gradually increased, reached a peak at 1 week after crush, and then returned to the normal level at 4 weeks. Besides, we observed that up-regulation of SKIP was approximately in parallel with Proliferating cell nuclear antigen (PCNA), and numerous Schwann cells (SCs) expressing SKIP were PCNA and Ki-67 positive. Collectively, we hypothesized peripheral nerve crush induced up-regulation of SKIP in the sciatic nerve, which was associated with SCs proliferation. |
| Databáze: | OpenAIRE |
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