Alteration of macrophage immune phenotype in a murine sepsis model is associated with susceptibility to secondary fungal infection
| Autor: | Vu Ctb, Patcharee Ritprajak, Asada Leelahavanichkul, Arsa Thammahong |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
T cell
Immunology Population Programmed Cell Death 1 Receptor Sepsis 03 medical and health sciences Mice Immune system medicine Immunology and Allergy Macrophage Animals Humans education Candida albicans B cell 030304 developmental biology CD86 0303 health sciences education.field_of_study biology 030306 microbiology business.industry Macrophages General Medicine medicine.disease biology.organism_classification Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure Phenotype Mycoses business |
| Zdroj: | Asian Pacific journal of allergy and immunology. 40(2) |
| ISSN: | 0125-877X |
| Popis: | Background Secondary fungal infection is a major complication in patients with sepsis-associated immunosuppression. However, sepsis-induced immune alterations related to fungal susceptibility have not been well characterized. Objectives To determine kinetic changes in the immune phenotype by determining the proportion of T cells, B cells and macrophages, and especially the expression of an immune exhaustion marker PD-1, in murine sepsis. In addition, sepsis -induced alterations of these immune cells were assessed in relation to susceptibility to secondary fungal infection. Methods Cecal ligation and puncture (CLP) was used as a mouse sepsis model, with Candida albicans as the secondary systemic fungal infection. Splenic T cells, B cells and macrophages were assessed by flow cytometry. Results Alterations in T cell and B cell numbers and the proportion of PD-1 expressing T cells and B cells in CLP mice were not clearly related to susceptibility to secondary Candida infection. By contrast, changes in levels of CD86+-activated macrophages, and the proportion of the PD-1+ population among the CD86+ macrophages in CLP mice were found to be related to secondary fungal infection susceptibility. Conclusions Macrophage activation and exhaustion might be a significant determinant in susceptibility to fungal infection, and outcomes of infection. This study provided more comprehensive knowledge pertinent to patient evaluation and therapeutics design in restoring host defenses against secondary fungal infection in those with sepsis. |
| Databáze: | OpenAIRE |
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