| Autor: |
Fatmagül İlayda Aydınlı, Sezgin Er, Bilal Ersen Kerman |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Journal of Neuroscience Methods. 382:109723 |
| ISSN: |
0165-0270 |
| Popis: |
Background: The plasticity of macrophages in the immune response is a dynamic situation dependent on external stimuli. The activation of macrophages both has beneficial and detrimental effects on mature oligodendrocytes (OLs) and myelin. The activation towards inflammatory macrophages has a critical role in the immune-mediated oligodendrocytes death in multiple sclerosis (MS) lesions. New method: We established an in vitro co-culture method to study the function of macrophages in the survival and maturation of OLs. Results: We revealed that M1 macrophages decreased the number of mature OLs and phagocytosed the myelin. Interestingly, non-activated as well as M2 macrophages contributed to an increase in the number of mature OLs in our in vitro co-culture platform. Comparison with existing methods: We added an antibody against an OL surface antigen in our in vitro co-cultures. The antibody presents the OLs to the macrophages enabling the investigation of direct interactions between macrophages and OLs. Conclusion: Our co-culture system is a feasible method for the investigation of the direct cell-to-cell interactions between OLs and macrophages. We utilized it to show that M2 and non-activated macrophages may be employed to enhance remyelination. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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