In vitro evaluation of the genotoxic and cytotoxic effects of artesunate, an antimalarial drug, in human lymphocytes
| Autor: | Regianne Maciel dos Santos Correa, P. P. B. Santana, Moysés dos Santos Miranda, Tatiane Cristina Mota, Marcelo de Oliveira Bahia, Rommel Rodríguez Burbano, Lorena Monteiro Gomes, Plínio Cerqueira dos Santos Cardoso, Priscilla Cristina Moura Vieira |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Epidemiology Health Toxicology and Mutagenesis Artemisia annua Artesunate Apoptosis Pharmacology medicine.disease_cause Antimalarials chemistry.chemical_compound medicine Humans Lymphocytes Artemisinin Cells Cultured Genetics (clinical) Micronucleus Tests biology fungi biology.organism_classification Artemisinins Comet assay chemistry Micronucleus test Female Comet Assay Micronucleus Genotoxicity medicine.drug |
| Zdroj: | Environmental and Molecular Mutagenesis. 52:590-594 |
| ISSN: | 0893-6692 |
| DOI: | 10.1002/em.20659 |
| Popis: | Artesunate is one of the main antimalarial drugs used in several countries. It is a semisynthetic compound derived from artemisinin, a substance extracted from the Chinese plant, Artemisia annua L. Despite the widespread use of artesunate as an antimalarial drug, there is a lack of data regarding its genotoxic effects in human lymphocytes. Therefore, in this study, we used the comet assay and micronucleus test to evaluate the possible genotoxic effects of artesunate in cultured human lymphocytes. In addition, cell death by necrosis and apoptosis was also assessed. Cells exposed to different concentrations of artesunate showed a significant concentration-dependent increase (P < 0.05) in DNA damage index and micronuclei frequency. A significant increase in the frequency of apoptotic and necrotic cells was also observed. Our results showed that artesunate is a genotoxic and cytotoxic compound in cultured human lymphocytes. Environ. Mol. Mutagen., 2011. © 2011 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text