Surface Expression of HLA-C Antigen by Human Extravillous Trophoblast
| Autor: | A. Ziegler, Yung Wai Loke, Ashley King, Sarah Joseph, P. Le Bouteiller, Lucy Gardner, B. Uchanska-Ziegler, Tanya D. Burrows, Susan E. Hiby, J.M. Bowen, Sanjay Verma, P.B. Lim |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
HLA-C Antigens Human leukocyte antigen Biology Interferon-gamma HLA-C Receptors KIR Antigen KIR2DL1 HLA Antigens Pregnancy Tumor Cells Cultured medicine Humans Interferon gamma Choriocarcinoma RNA Messenger Receptors Immunologic Fluorescent Antibody Technique Indirect HLA-G Antigens Beta-2 microglobulin Histocompatibility Antigens Class I Obstetrics and Gynecology Trophoblast Cytotoxicity Tests Immunologic Flow Cytometry Molecular biology Recombinant Proteins Trophoblasts Up-Regulation Killer Cells Natural medicine.anatomical_structure Reproductive Medicine Antigens Surface Receptors KIR2DL1 embryonic structures Electrophoresis Polyacrylamide Gel Female beta 2-Microglobulin Developmental Biology medicine.drug |
| Zdroj: | Placenta. 21:376-387 |
| ISSN: | 0143-4004 |
| DOI: | 10.1053/plac.1999.0496 |
| Popis: | In this paper definitive evidence that the classical class I product, HLA-C, is expressed on the surface of normal trophoblast cells is provided. HLA-C transcripts were sequenced from cDNA isolated from first trimester trophoblast cells obtained by flow cytometric sorting. Both paternal and maternal alleles were transcribed. HLA-C proteins were demonstrated by biochemical analysis and found on the cell surface in association with beta(2)-microglobulin. Upregulation of cell surface HLA-C but not HLA-G expression after interferon (IFN)-gamma treatment was demonstrated by flow cytometric analysis. Immunohistology has confirmed HLA-C is expressed by all extravillous subpopulations in vivo. The question of whether trophoblast HLA-C molecules interact with decidual NK cells expressing killer Ig-like receptors (KIR) has also been addressed. Our results demonstrate that extravillous trophoblast expresses at least two HLA class I molecules, HLA-G and HLA-C on the cell surface. |
| Databáze: | OpenAIRE |
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