Functional Analysis of the Arabidopsis thaliana CDPK-Related Kinase Family: AtCRK1 Regulates Responses to Continuous Light

Autor: János Urbancsok, Laura Zsigmond, Ildikó Valkai, Abu Imran Baba, Klaus Palme, Norbert Andrási, Ferhan Ayaydin, Taras Pasternak, Gábor Rigó, Ágnes Cséplő, László Szabados, Ateeq Ur Rehman, Imre Vass
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
Chlorophyll
Arabidopsis thaliana
Arabidopsis
Cellular homeostasis
01 natural sciences
Plant Roots
singlet oxygen
lcsh:Chemistry
Adapter molecule crk
Gene Expression Regulation
Plant
gravitropic response
Photosynthesis
lcsh:QH301-705.5
Spectroscopy
biology
Chemistry
plasma membrane localization
Gene Expression Regulation
Developmental
General Medicine
Hypocotyl
Computer Science Applications
Cell biology
cell death
Phenotype
Sunlight
CDPK-related kinase (CRK) family
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Endomembrane system
RNA
Messenger
Physical and Theoretical Chemistry
Protein kinase A
Molecular Biology
Arabidopsis Proteins
Organic Chemistry
Calcium-Binding Proteins
Cell Membrane
Wild type
biology.organism_classification
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Mutation
Polar auxin transport
Protein Kinases
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences; Volume 19; Issue 5; Pages: 1282
International Journal of Molecular Sciences, Vol 19, Iss 5, p 1282 (2018)
ISSN: 1422-0067
Popis: The Calcium-Dependent Protein Kinase (CDPK)-Related Kinase family (CRKs) consists of eight members in Arabidopsis. Recently, AtCRK5 was shown to play a direct role in the regulation of root gravitropic response involving polar auxin transport (PAT). However, limited information is available about the function of the other AtCRK genes. Here, we report a comparative analysis of the Arabidopsis CRK genes, including transcription regulation, intracellular localization, and biological function. AtCRK transcripts were detectable in all organs tested and a considerable variation in transcript levels was detected among them. Most AtCRK proteins localized at the plasma membrane as revealed by microscopic analysis of 35S::cCRK-GFP (Green Fluorescence Protein) expressing plants or protoplasts. Interestingly, 35S::cCRK1-GFP and 35S::cCRK7-GFP had a dual localization pattern which was associated with plasma membrane and endomembrane structures, as well. Analysis of T-DNA insertion mutants revealed that AtCRK genes are important for root growth and control of gravitropic responses in roots and hypocotyls. While Atcrk mutants were indistinguishable from wild type plants in short days, Atcrk1-1 mutant had serious growth defects under continuous illumination. Semi-dwarf phenotype of Atcrk1-1 was accompanied with chlorophyll depletion, disturbed photosynthesis, accumulation of singlet oxygen, and enhanced cell death in photosynthetic tissues. AtCRK1 is therefore important to maintain cellular homeostasis during continuous illumination. © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Databáze: OpenAIRE
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