Proteomic and biological profiling of extracellular vesicles from Alzheimer's disease human brain tissues
| Autor: | Zhi Ruan, Satoshi Muraoka, Jina Ko, Yang You, David Issadore, Howard E. Gendelman, Yuzhi Wang, Joseph Zaia, Kayo Yukawa-Takamatsu, Annina M. DeLeo, John D. Hogan, Manveen K. Sethi, Tsuneya Ikezu, Mei Chen, Maria Medalla, Zijian Yang, Seiko Ikezu, Santhi Gorantla, Weiming Xia |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Proteomics 0301 basic medicine Proteome Epidemiology Amyloid beta Quantitative proteomics tau Proteins Disease Extracellular vesicles Article Machine Learning Extracellular Vesicles 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Developmental Neuroscience Alzheimer Disease medicine Humans Phosphorylation 030304 developmental biology Aged Aged 80 and over 0303 health sciences Amyloid beta-Peptides biology Health Policy Brain Human brain Cortical grey matter 3. Good health Brain disease Cell biology Psychiatry and Mental health 030104 developmental biology medicine.anatomical_structure biology.protein Female Neurology (clinical) Neuron Geriatrics and Gerontology 030217 neurology & neurosurgery |
| Zdroj: | Alzheimers Dement |
| ISSN: | 1552-5279 1552-5260 |
| DOI: | 10.1002/alz.12089 |
| Popis: | IntroductionExtracellular vesicles (EVs) from human Alzheimer’s disease (AD) biospecimens contain amyloid-β peptide (Aβ) and tau. While AD EVs are known to affect brain disease pathobiology, their biochemical and molecular characterizations remain ill defined.MethodsEVs were isolated from the cortical grey matter of 20 AD and 18 control brains. Tau and Aβ levels were measured by immunoassay. Differentially expressed EV proteins were assessed by quantitative proteomics and machine learning.ResultsLevels of pS396 tau and Aβ were significantly elevated in AD EVs. High levels of neuron- and glia- specific factors are detected in control and AD EVs, respectively. Machine learning identified ANXA5, VGF, GPM6A and ACTZ in AD EV compared to controls. They distinguished AD EVs from controls in the test sets with 88% accuracy.DiscussionIn addition to Aβ and tau, ANXA5, VGF, GPM6A and ACTZ are new signature proteins in AD EVs. |
| Databáze: | OpenAIRE |
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