| Autor: |
Sharon Karniely, Adi Faigenboim, Salsabeel Watted, Katia Lapin, Eduard Berenshtein, Avshalom Hurvitz, Arieli Bouznach, Ezra Rozenblut, Massimo Orioles, Marco Galeotti, Irene Salinas, Asaf Berkowitz, Eran Bacharach, Avi Eldar |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
iScience |
| ISSN: |
2589-0042 |
| Popis: |
Rainbow trout (Oncorhynchus mykiss) is the principal species of inland-farmed fish in the Western hemisphere. Recently, we diagnosed in farmed rainbow trout a disease in which the hallmark is granulomatous-like hepatitis. No biotic agents could be isolated from lesions. Still, unbiased high-throughput sequencing and bioinformatics analyses revealed the presence of a novel piscine nidovirus that we named “Trout Granulomatous Virus” (TGV). TGV genome (28,767 nucleotides long) is predicted to encode non-structural (1a and 1 ab) and structural (S, M, and N) proteins that resemble proteins of other known piscine nidoviruses. High loads of TGV transcripts were detected by quantitative RT-PCR in diseased fish and visualized in hepatic granulomatous sites by fluorescence in situ hybridization. Transmission electron microscopy (TEM) revealed coronavirus-like particles in these lesions. Together, these analyses corroborated the association of TGV with the lesions. The identification and detection of TGV provide means to control TGV spread in trout populations. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
