Centrosomal protein FOR20 knockout mice display embryonic lethality and left-right patterning defects
Autor: | Shanshan Xie, Tianhua Zhou, Xiaoyi Yan, Wenjun Kuang, Bai Ge, Xiying Chen, Min Liu, Cheng Gao, Zhangqi Xu, Feifei Liu |
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Rok vydání: | 2021 |
Předmět: |
Male
Heterozygote Biophysics Embryonic Development Biology Biochemistry 03 medical and health sciences Mice Structural Biology Ciliogenesis Genetics Animals Cilia RNA Messenger Molecular Biology 030304 developmental biology Body Patterning Mice Knockout 0303 health sciences Neovascularization Pathologic Cilium 030302 biochemistry & molecular biology Embryogenesis Homozygote Gene targeting Cell migration Embryo Cell Biology Embryo Mammalian Embryonic stem cell Cell biology Knockout mouse Embryo Loss Female Gene Deletion |
Zdroj: | FEBS lettersReferences. 595(10) |
ISSN: | 1873-3468 |
Popis: | Centrosomal protein FOR20 has been reported to be crucial for essential cellular processes, including ciliogenesis, cell migration, and cell cycle in vertebrates. However, the function of FOR20 during mammalian embryonic development remains unknown. To investigate the in vivo function of the For20 gene in mammals, we generated For20 homozygous knockout mice by gene targeting. Our data reveal that homozygous knockout of For20 results in significant embryonic growth arrest and lethality during gestation, while the heterozygotes show no obvious defects. The absence of For20 leads to impaired left-right patterning of embryos and reduced cilia in the embryonic node. Deletion of For20 also disrupts angiogenesis in yolk sacs and embryos. These results highlight a critical role of For20 in early mammalian embryogenesis. |
Databáze: | OpenAIRE |
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