Evaluating the influence of progesterone concentration and time of exposure on in vitro endometrial decidualisation
| Autor: | Sutham Suthaporn, Marcos Castellanos, Kate F. Walker, Sean T. May, Juan H. Hernandez-Medrano, Jim G Thornton, Kanna Jayaprakasan, Walid E. Maalouf |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Time Factors Stromal cell Microarray Down-Regulation 030209 endocrinology & metabolism Endometrium Biochemistry Insulin-like growth factor-binding protein Cell Line Incubation period Andrology 03 medical and health sciences 0302 clinical medicine Endocrinology Exome Sequencing medicine Humans Gene Regulatory Networks IGFBP1 Molecular Biology Progesterone Oligonucleotide Array Sequence Analysis Dose-Response Relationship Drug biology Chemistry Gene Expression Profiling Cell Cycle In vitro Insulin-Like Growth Factor Binding Protein 1 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Cell culture biology.protein Female Stromal Cells |
| ISSN: | 0303-7207 1872-8057 |
| Popis: | This study aimed to evaluate the influence of progesterone (concentration and time of exposure) on endometrial decidualisation using an in vitro model cell line: Human Endometrial Stromal Cells (HESCs). HESCs exposed to progesterone (1 and 10 μM) had higher percentages of decidualised cells and higher expression of the decidual marker (Insulin Like Growth Factor Binding Protein 1 (IGFBP1)) compared with those exposed to (0.1 μM). Among those HESCs cultured with 1 μM progesterone for 11 days, the highest rate of morphological differentiation (40–50%) occurred between days 7–9 and IGFBP1 peaked on day 7. The cell-cycle pathway was significantly down-regulated in HESCs exposed to at least 1 μM progesterone regardless of the incubation period. We conclude that exposure to high progesterone concentration for 7–9 days is essential to maximise the process of decidualisation. |
| Databáze: | OpenAIRE |
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