The cytoskeletal protein villin as a parameter for early detection of tubular damage in the human kidney
| Autor: | L.B. Zimmerhackl, B. Leuk, H. Hoschützky |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Sodium
Lumen (anatomy) chemistry.chemical_element macromolecular substances Urine digestive system Biochemistry Analytical Chemistry Antigen medicine Humans Child Cytoskeleton Kidney Chromatography biology Chemistry Calcium-Binding Proteins Microfilament Proteins Organic Chemistry General Medicine Kidney Transplantation Molecular biology Molecular Weight Blot Kidney Tubules medicine.anatomical_structure Chromatography Gel biology.protein Electrophoresis Polyacrylamide Gel Kidney Diseases Carrier Proteins Villin |
| Zdroj: | Journal of Chromatography A. 587:81-84 |
| ISSN: | 0021-9673 |
| Popis: | Villin is a cytoskeletal protein of brush borders in the kidney and gut. After renal tubular cell injury the brushborder fragments are shedded into the tubular lumen and excreted with urine indicating renal tubular damage (so called “renal antigen” shedding). In urine villin appears as intact molecule (95 000 dalton) and as fragment with 70 000, 45 000 and 22 000 dalton. The major villin fragment (70 000 dalton) was purified after ammonium sulphate precipitation from urine of human renal transplant recipients. Final purification of the villin 70 000 dalton fragment was achieved by gel filtration with TSK 3000 SWG preparative grade. Purification was varified by sodium dodecyl sulphate-polyacrylamide gel eletrophoresis and western blotting. |
| Databáze: | OpenAIRE |
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