Transverse pore gradient gel electrophoresis, using the PhastSystem
| Autor: | Mark M. Garner, Andreas Chrambach, David L. Wheeler, Zsuzsanna Buzás, Dietmar Tietz |
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| Rok vydání: | 1994 |
| Předmět: |
Gel electrophoresis
Pore size Base Composition Chromatography Materials science Clinical Biochemistry Analytical chemistry Small sample DNA Biochemistry Analytical Chemistry Staining Molecular Weight Electrophoresis Transverse plane Electrophoresis Polyacrylamide Gel Indicators and Reagents Short duration Software |
| Zdroj: | Electrophoresis. 15(8-9) |
| ISSN: | 0173-0835 |
| Popis: | The application of pore gradient gels prefabricated for the PhastSystem (Pharmacia) to transverse pore gradient gel electrophoresis is demonstrated. It has the twofold advantage of (i) horizontal positioning, avoiding gel stretching during the preparation of these gels and resulting pore size irreproducibility experienced with vertically applied pore gradient gels, which necessitate an orthogonal transfer of spacers, and (ii) miniaturized gel dimensions, which allow a small sample load and a short duration of electrophoresis and staining. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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