Evaluation of the Activities of Concentrated Crude Mannan-degrading Enzymes Produced by Aspergillus niger
| Autor: | M. Rosfarizan, M. Sitis Norita, A. B. Ariff |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Malaysian Journal of Microbiology, Vol 6, Iss 2, Pp 171-180 (2010) |
| ISSN: | 2231-7538 1823-8262 |
| Popis: | The mannan-degrading enzymes produced by Aspergillus niger were concentrated and the activities were evaluated. The optimum pH for β-mannanase, endoglucanase and α-galactosidase were obtained at pH 3.5 while pH optimum for β-mannosidase was occurred at pH 3.0. The β-mannanase, endoglucanase, β-mannosidase and α-galactosidase was stable at pH 3.5 to 7, pH 3.5 to 6.5, pH 4 to 7 and pH 3.5 to 5.0, respectively. The enzymes obtained in this study were characterized and defined as acidic proteins. The β-mannanases from A. niger had two optimum temperatures (at 50 °C and 60 °C) and its half-life was 6 h and 4 h at 60 °C and 70 °C, respectively. The β-mannosidase, endoglucanase and α-galactosidase displayed optimal activity at 70 °C, 60 °C and 50 – 60 °C, respectively. The β-mannosidase had half-life of 1.5 h at 70 °C, while α-galactosidase had a half-life of 2.5 h at 60 °C and endoglucanase had a half-life of 6 h at 60 °C and 45 min at 70 °C. |
| Databáze: | OpenAIRE |
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