Ginsenoside Rg1 Inhibits Cell Proliferation and Induces Markers of Cell Senescence in CD34+CD38- Leukemia Stem Cells Derived from KG1α Acute Myeloid Leukemia Cells by Activating the Sirtuin 1 (SIRT1)/Tuberous Sclerosis Complex 2 (TSC2) Signaling Pathway
| Autor: | Cheng-Gui Zhang, Yaping Wang, Yan-Jun Han, Yuan Li, Yan-Long Tang, Heng Liu, Cui-Li Wang, Yue Zhou |
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| Rok vydání: | 2020 |
| Předmět: |
Myeloid
Ginsenosides Cell Survival Cell Down-Regulation Antigens CD34 030204 cardiovascular system & hematology CD38 03 medical and health sciences 0302 clinical medicine Sirtuin 1 Tuberous Sclerosis Lab/In Vitro Research hemic and lymphatic diseases Cell Line Tumor medicine Biomarkers Tumor Humans Cellular Senescence Cell Proliferation Cell growth Chemistry beta-Glucosidase Cell Cycle Myeloid leukemia General Medicine Cell cycle medicine.disease ADP-ribosyl Cyclase 1 Leukemia Leukemia Myeloid Acute Adult Stem Cells medicine.anatomical_structure 030220 oncology & carcinogenesis Cancer research Neoplastic Stem Cells Stem cell Signal Transduction |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| Popis: | BACKGROUND Clinical relapse in acute myeloid leukemia (AML) is associated with the reduced treatment response of leukemia stem cells (LSCs). This study aimed to investigate the effects of the ginseng derivative, ginsenoside Rg1 (Rg1), on CD34+CD38- LSCs derived from KG1a human acute myeloid leukemia cells. MATERIAL AND METHODS CD34+CD38- LSCs were isolated from KG1a human acute myeloid leukemia cells by cell sorting. CD34+CD38- KG1alpha LSCs were divided into the control group and the Rg1 group (treated with Rg1). The cell counting kit-8 (CCK-8) assay evaluated the proliferation of CD34+CD38- KG1alpha LSCs and flow cytometry studied the cell cycle. The mixed colony-forming unit (CFU-Mix) assay and staining for senescence-associated beta-galactosidase (SA-s-Gal) evaluated cell senescence. Expression of sirtuin 1 (SIRT1) and tuberous sclerosis complex 2 (TSC2) were evaluated using Western blot and quantitative reverse transcription-polymerase chain reaction (qRT-PCR). RESULTS CD34+CD38- KG1alpha LSCs were isolated at 98.72%. Rg1 significantly reduced the proliferation of CD34+CD38- KG1alpha LSCs compared with the control group (p |
| Databáze: | OpenAIRE |
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