Are vaginal swabs comparable to cervical smears for human papillomavirus DNA testing?

Autor: Jerina Boelens, Jo Van Dorpe, Marleen Praet, Ans Traen, Elizaveta Padalko, Liselotte Coorevits, Luc Bingé
Rok vydání: 2018
Předmět:
Uterine Cervical Neoplasms
Cervix Uteri
medicine.disease_cause
Cervix
Human Papillomavirus DNA Tests
0302 clinical medicine
Medicine and Health Sciences
030212 general & internal medicine
Papillomaviridae
Human Papillomavirus DNA Test
Early Detection of Cancer
Trichomonas Vaginalis
biology
medicine.diagnostic_test
Obstetrics
HPV infection
WOMEN
virus diseases
Obstetrics and Gynecology
General Medicine
Middle Aged
SELF
female genital diseases and pregnancy complications
PREVALENCE
Squamous intraepithelial lesion
medicine.anatomical_structure
CHLAMYDIA-TRACHOMATIS
Chlamydia Trachomatis
Oncology
Neisseria Gonorrhoeae
030220 oncology & carcinogenesis
Vagina
Mycoplasma Genitalium
Sexually Transmitted Diseases
Female
Original Article
Squamous Intraepithelial Lesions of the Cervix
Papanicolaou Test
Adult
medicine.medical_specialty
Cervical intraepithelial neoplasia
Sensitivity and Specificity
03 medical and health sciences
Virology
medicine
Humans
FEMALE SEX WORKERS
SEXUALLY-TRANSMITTED INFECTIONS
Pelvic examination
METAANALYSIS
TRICHOMONAS-VAGINALIS
Vaginal Smears
Sex Workers
business.industry
Papillomavirus Infections
Uterine Cervical Dysplasia
biology.organism_classification
medicine.disease
Molecular Typing
DNA
Viral
Trichomonas vaginalis
business
CLINICIAN
HPV INFECTION
Zdroj: Journal of Gynecologic Oncology
JOURNAL OF GYNECOLOGIC ONCOLOGY
ISSN: 2005-0399
2005-0380
DOI: 10.3802/jgo.2018.29.e8
Popis: Objective: Human papillomavirus (HPV) testing is widely incorporated into cervical cancer screening strategies. Current screening requires pelvic examination for cervical sampling, which may compromise participation. The acceptance could be raised by introducing testing on vaginal swabs. We explored the interchangeability of vaginal swabs and cervical smears for HPV testing, by means of a prospective study conducted in female sex workers (FSWs). Besides, we report on the occurrence of 32 different HPV genotypes in FSW with low-grade squamous intraepithelial lesion (LSIL) or high-grade squamous intraepithelial lesion (HSIL). Methods: Paired physician-collected vaginal swabs and cervical smears from 303 FSW were tested for HPV using the Abbott RealTime High-Risk HPV assay. Cervical cytology was examined on cervical smears. In case of HSIL/LSIL cytological classification (n=52), both samples were genotyped using INNO-LiPa HPV Genotyping Extra II. Results: The overall prevalence of high-risk (HR)-HPV was 51%. In FSW with HSIL/LSIL cervical cytology, the sensitivity and specificity of vaginal samples for the detection of HRHPV was 100% and 70% and for probable HR-HPV 100% and 91%. The mean number of genotypes identified in vaginal samples (mean=3.5; 95% confidence interval [CI]=2.8-4.2) was significantly higher than in cervical smear samples (mean=2.6; 95% CI=2.1-3.0) (p=0.001). The most frequently encountered HR-HPV genotypes were HPV16, 31, 51, and 52. Conclusion: As our study shows that vaginal swabs are equivalent to cervical smears for the detection of (probable) HR-HPV, vaginal swabs can be used for HPV testing in cervical cancer screening strategies. Given the acceptance of vaginal sampling, this finding offers an opportunity to boost screening coverage. The Fonds Wetenschappelijk Onderzoek-Vlaanderen (FWO) has granted this study, embedded in a PhD Clinical Fellowship with application number 1700417N.
Databáze: OpenAIRE
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