Gonadectomy-related adrenocortical tumors in ferrets demonstrate increased expression of androgen and estrogen synthesizing enzymes together with high inhibin expression
| Autor: | de Jong, M K, ten Asbroek, E E M, Sleiderink, A J, Conley, A J, Mol, J A, Schoemaker, N J, Onderzoek, LS Vet. Fysiologie en Anatomie, Vogels en bijzondere dieren, CSCA TR2, CSCA AVM, Tissue Repair, Advances in Veterinary Medicine |
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| Rok vydání: | 2014 |
| Předmět: |
Male
endocrine system medicine.medical_specialty medicine.drug_class Adrenal Gland Neoplasms Biology Polymerase Chain Reaction Aromatase Endocrinology Food Animals Internal medicine Gene expression medicine Animals Inhibins Adrenocortical tumor Neoplastic Steroidogenic acute regulatory protein Carcinoma Androstenedione Ferrets Estrogens Androgen Immunohistochemistry Gene Expression Regulation Neoplastic Real-time polymerase chain reaction Gene Expression Regulation Estrogen Androgens biology.protein Female Animal Science and Zoology Immunostaining |
| Zdroj: | Domestic Animal Endocrinology, 48. Elsevier |
| ISSN: | 0739-7240 |
| Popis: | The 2 objectives of this study were to (1) measure by quantitative polymerase chain reaction the expression of genes involved in steroid and inhibin synthesis in adrenocortical tumors of gonadectomized ferrets and (2) localize by immunohistochemistry several proteins that are key to adrenal steroidogenesis. Relative to the control adrenals, expression of the messenger RNAs encoding StAR (steroidogenic acute regulatory protein; P = 0.039), CYP11A (P = 0.019), CYP21 (P = 0.01), and 3β-HSD (P = 0.004), all involved in the synthesis of mineralocorticoids and glucocorticoids, were decreased in the adrenocortical tumors. In contrast, expression of cytochrome B5 (CytB5; P = 0.0001) and aromatase (P = 0.003), involved in androgen and estrogen synthesis, and both inhibin α-subunit (P = 0.002) and βB-subunit (P = 0.001) were upregulated. In tumors, immunostaining of CYP21 was low, whereas staining of Cyp17 and CytB5, necessary for androgen synthesis, was present. It is concluded that ferret adrenocortical tumors express genes for androgen production. In addition, the expression of aromatase and inhibin suggests an even more gonadal differentiation, which is reminiscent to the fact that both gonads and adrenals are derived from a common urogenital primordial cell. |
| Databáze: | OpenAIRE |
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