Highly selective fluorescence ‘turn off’ sensing of picric acid and efficient cell labelling by water-soluble luminescent anthracene-bridged poly(N-vinyl pyrrolidone)
| Autor: | Archana Kumari, Pralay Maiti, Rajshree Singh, Shikha Singh, Nira Misra, Biswajit Ray, Sambhav Vishwakarma, Jaydeep Singh, Sudipta Senapati, Susanta K. Sen Gupta, Kheyanath Mitra, Vijay Kumar Patel |
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| Rok vydání: | 2019 |
| Předmět: |
Anthracene
Quenching (fluorescence) biology 010401 analytical chemistry Picric acid 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry Fluorescence Fluorescence spectroscopy 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound chemistry Electrochemistry biology.protein Fluorescence microscope Click chemistry Environmental Chemistry Bovine serum albumin 0210 nano-technology Spectroscopy Nuclear chemistry |
| Zdroj: | The Analyst. 144:3620-3634 |
| ISSN: | 1364-5528 0003-2654 |
| DOI: | 10.1039/c8an02417k |
| Popis: | A novel, water-soluble, luminescent anthracene-bridged AA-type bi-arm poly(N-vinylpyrrolidone) (ATC-PNVP) was synthesized using a click reaction between alkyne-terminated PNVP and 9,10-bis(azidomethyl)anthracene. The resultant anthracene-bridged PNVP (ATC-PNVP) was characterized using 1H NMR, FTIR, UV-Vis, and fluorescence spectroscopic methods and GPC analysis. ATC-PNVP showed effective fluorescence properties in an aqueous medium. It showed highly selective "turn off" sensing behaviour towards picric acid, a common nitro-aromatic explosive, with a wide linear range of detection of 0.01-0.3 mM and LOD value of 0.006 mM in water. ATC-PNVP-based paper sensors also showed very effective detection of picric acid in the concentration range 0.001-1.0 mM. Its binding with bovine serum albumin (BSA) was studied using steady-state, synchronous and 3D fluorescence spectroscopy and this study showed effective quenching of the intrinsic fluorescence of BSA and occurrence of a FRET-type interaction. Furthermore, this luminescent ATC-PNVP was efficiently used as a fluorescence microscopy labelling agent in NIH-3T3 and HeLa cells, and showed greater uptake and hence better fluorescent labelling in the cytosols of the tested cells than free 9,10-bis(azidomethyl) anthracene. The cell viability study also showed a very good biocompatible and non-toxic nature of ATC-PNVP at lower working concentrations towards each of the types of cells tested. |
| Databáze: | OpenAIRE |
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