Endozoicomonas numazuensis sp. nov., a gammaproteobacterium isolated from marine sponges, and emended description of the genus Endozoicomonas Kurahashi and Yokota 2007
| Autor: | Atsuko Katsuta, Miyuki Nishijima, Kazuhide Yamasato, Kyoko Adachi, Yoshikazu Shizuri |
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| Rok vydání: | 2012 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Microbiology Japan Genus Phylogenetics RNA Ribosomal 16S Botany Gammaproteobacteria Carbohydrate fermentation Animals Ecology Evolution Behavior and Systematics Phylogeny Base Composition biology Fatty Acids Quinones General Medicine Marine invertebrates Sequence Analysis DNA Ribosomal RNA biology.organism_classification 16S ribosomal RNA Halophile Bacterial Typing Techniques Porifera |
| Zdroj: | International journal of systematic and evolutionary microbiology. 63(Pt 2) |
| ISSN: | 1466-5034 |
| Popis: | Two non-motile, rod-shaped gammaproteobacteria were isolated from marine sponges collected from the coast of Japan at Numazu. The isolates were oxidase- and catalase-positive facultative anaerobes that fermented carbohydrates. They required sodium ions for growth and were slightly halophilic, growing in the presence of 1.0–5.0 % (w/v) NaCl (optimum of 2.0 % NaCl). Under aerobic conditions, the major isoprenoid quinones were ubiquinone-9 and menaquinone-9 and the minor quinones were ubiquinone-8 and menaquinone-8. The major cellular fatty acids were C18 : 1ω7c, C16 : 1ω7c and C16 : 0 and the hydroxy acids were C10 : 0 3-OH and C12 : 0 3-OH. The DNA G+C content was 48.3–48.7 mol%. Phylogenetic analysis of 16S rRNA gene sequences placed the isolates within the radiation of the genus Endozoicomonas in a broad clade of uncultured clones recovered from various marine invertebrates. The isolates exhibited 96.5–96.9 % 16S rRNA gene sequence similarity with Endozoicomonas elysicola MKT110T and Endozoicomonas montiporae CL-33T, with which the isolates formed a monophyletic cluster with 100 % bootstrap support. The phenotypic features (carbohydrate fermentation, quinone system and some major cellular fatty acids) differed from those of members of the genus Endozoicomonas , which are aerobic, produce little or no menaquinone under aerobic conditions and possess different amounts of C14 : 0 and C18 : 1ω7c. Although some phenotypic differences were identified, the isolates should be assigned to the genus Endozoicomonas on the basis of congruity of phylogeny and should be classified as representatives of a novel species, for which the name Endozoicomonas numazuensis sp. nov. is proposed. The type strain is HC50T ( = NBRC 108893T = DSM 25634T). An emended description of the genus Endozoicomonas is presented. |
| Databáze: | OpenAIRE |
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