New immunoassay for the mapping of neurofibrillary degeneration in Alzheimer's disease using two monoclonal antibodies against human paired helical filament tau proteins
| Autor: | Valérie Buée-Scherrer, A. Wattez, Olivier Condamines, André Delacourte, Laurence Boissier, Chantal Mourton-Gilles, Bernard Pau |
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| Rok vydání: | 1995 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.drug_class Enzyme-Linked Immunosorbent Assay tau Proteins Biology Monoclonal antibody Epitope Mice Degenerative disease Alzheimer Disease mental disorders medicine Animals Humans Aged Aged 80 and over Mice Inbred BALB C medicine.diagnostic_test General Neuroscience Antibodies Monoclonal Brain medicine.disease Immunoassay Nerve Degeneration Monoclonal Neurofibrils biology.protein Phosphorylation Antibody Alzheimer's disease |
| Zdroj: | Neuroscience Letters. 192:81-84 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/0304-3940(95)11617-6 |
| Popis: | Monoclonal antibodies against human paired helical filament tau (PHF-tau) proteins were produced. Two of these antibodies, AD1 and AD2, were shown by immunoblot to be directed against distinct hyperphosphorylated epitopes of the PHF-tau proteins. Using AD1 and AD2, an antigen-capture ELISA specific for PHF-tau proteins was developed and used to map the neurofibrillary degeneration of several Broadmann areas from an Alzheimer's disease patient. The results confirm that the neurofibrillary degeneration predominates in parietal and temporal lobes. |
| Databáze: | OpenAIRE |
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