Platinum-Based Drugs Differentially Affect the Ultrastructure of Breast Cancer Cell Types
| Autor: | Mohamed A. Al-Kindi, Abdurahman Al-Nabhani, Kawther Al-Adawi, Shadia Al-Bahlani, Buthaina Al-Dhahli |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Article Subject Organoplatinum Compounds Cell lcsh:Medicine Apoptosis Breast Neoplasms Biology General Biochemistry Genetics and Molecular Biology Carboplatin Cell membrane 03 medical and health sciences 0302 clinical medicine Microscopy Electron Transmission Cell Line Tumor Phagosomes Organelle medicine Humans Fragmentation (cell biology) Cisplatin General Immunology and Microbiology lcsh:R General Medicine Cell biology Oxaliplatin 030104 developmental biology medicine.anatomical_structure Cell culture Drug Resistance Neoplasm 030220 oncology & carcinogenesis Ultrastructure Female medicine.drug Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2017 (2017) |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | Breast cancer (BC) is the most common cause of cancer-related death worldwide. Although platinum-based drugs (PBDs) are effective anticancer agents, responsive patients eventually become resistant. While resistance of some cancers to PBDs has been explored, the cellular responses of BC cells are not studied yet. Therefore, we aim to assess the differential effects of PBDs on BC ultrastructure. Three representative cells were treated with different concentrations and timing of Cisplatin, Carboplatin, and Oxaliplatin. Changes on cell surface and ultrastructure were detected by scanning (SEM) and transmission electron microscope (TEM). In SEM, control cells were semiflattened containing microvilli with extending lamellipodia while treated ones were round with irregular surface and several pores, indicating drug entry. Prolonged treatment resembled distinct apoptotic features such as shrinkage, membrane blebs, and narrowing of lamellipodia with blunt microvilli. TEM detected PBDs’ deposits that scattered among cellular organelles inducing structural distortion, lumen swelling, chromatin condensation, and nuclear fragmentation. Deposits were attracted to fat droplets, explained by drug hydrophobic properties, while later they were located close to cell membrane, suggesting drug efflux. Phagosomes with destructed organelles and deposits were detected as defending mechanism. Understanding BC cells response to PBDs might provide new insight for an effective treatment. |
| Databáze: | OpenAIRE |
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