Dual emission nonionic molecular imprinting conjugated polythiophenes-based paper devices and their nanofibers for point-of-care biomarkers detection
| Autor: | Mirkomil Sharipov, Shavkatjon Azizov, Mohamed R. Elmasry, Chang Hyun Lee, Salah M. Tawfik, Yong-Ill Lee |
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| Rok vydání: | 2020 |
| Předmět: |
Paper
Polymers Nanofibers Biomedical Engineering Biophysics Nanotechnology Biosensing Techniques Thiophenes 02 engineering and technology Conjugated system 01 natural sciences Carcinoembryonic antigen Molecularly Imprinted Polymers Limit of Detection Neoplasms Biomarkers Tumor Electrochemistry Humans Saliva Point of care Detection limit biology Chemistry Dual emission 010401 analytical chemistry General Medicine 021001 nanoscience & nanotechnology Carcinoembryonic Antigen 0104 chemical sciences Point-of-Care Testing Nanofiber biology.protein Cancer biomarkers Smartphone alpha-Fetoproteins 0210 nano-technology Molecular imprinting Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 160:112211 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2020.112211 |
| Popis: | Enzyme-based assays have been extensively used for the early diagnosis of disease-related biomarkers. However, these assays are time-consuming, resource-intensive, and infrastructure-dependent, which renders them unsuitable and impractical for use in resource-constrained areas. Thus, there is a strong demand for a biocompatible and potentially generalizable sensor that can rapidly detect cancer biomarkers at ultralow concentration. Herein, an enzyme-free, cost-efficient, and easy-to-use assay based on a novel approach that entails fluorescent molecularly imprinting conjugated polythiophenes (FMICPs) for cancer biomarkers detection is developed. The promising conjugated polythiophenes structure, with a PLQY as high as 55%, provides a straightforward, and affordable method for free-enzyme signal generation. More importantly, the feasibility of integrating printed-paper technology with a sensitive and cost-effective smartphone and portable prototype testing device that could be utilized for rapid point-of-care (POC) cancer diagnostics is successfully introduced. Significantly, the unique structure of FMICP nanofibers (FMICP NFs) displays superior performance with enhanced sensitivity that is 80 times higher than that of pristine FMICP. This assay could lower the limits of detection to 15 fg mL−1 and 3.5 fg mL−1 for α-fetoprotein (AFP) and carcinoembryonic antigen (CEA), respectively, which are three orders of magnitude exceeding that of the standard enzyme-based assay. Moreover, the developed sensors are successfully applied to the fast diagnosis of AFP in liver cancer patients and the FMICP and FMICP NFs results are in excellent agreement with those of clinical ELISA. |
| Databáze: | OpenAIRE |
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